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Lung cancer strain with high potential power of bone transference and its generation

A technology of lung adenocarcinoma cell line and bone metastasis, applied in the field of lung adenocarcinoma cell line and its establishment, to achieve the effect of high short-term stagnation rate, high specificity and rapid cell growth

Inactive Publication Date: 2007-05-23
SHANGHAI CHEST HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There is no report on the use of radioisotope bone scintigraphy to find bone metastases in animal models of bone metastases in domestic and foreign literature

Method used

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  • Lung cancer strain with high potential power of bone transference and its generation
  • Lung cancer strain with high potential power of bone transference and its generation
  • Lung cancer strain with high potential power of bone transference and its generation

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1. Pre-experiment Human lung adenocarcinoma cell (SPC-A-1) 1×10 6 , injected under the skin of immunodeficiency mice, and when the tumor grew to 0.5-1 cm, the tumor was taken out, and the surrounding tumor tissue was cut into pieces, mashed, and cell cultured.

[0040] 2. Immunodeficiency mice weighing more than 20 grams, male or female. After purchasing, they were housed in SPF cages for several days.

[0041] 3. Anesthetize the experimental animals Inject diluted thiopental sodium and ketamine into the abdominal cavity and leg muscles of the mice respectively,

[0042] 4. Injection of cancer cells Inject 0.7×10 6 Human lung adenocarcinoma cells were cultured in SPF grade cages.

[0043] 5. Weighing Weigh the next day after two weeks of injection. Those weighing less than 16 grams should be closely observed to prevent death.

[0044] 6. Imaging Perform isotope bone scans every two weeks to find bone metastases, and select bone materials that meet the characteristi...

Embodiment 2

[0049] 1. The primary cultured bone metastasis cells after the left ventricle injection of cancer cells in the left ventricle of immunodeficient mice were used for future use.

[0050] 2. Immunodeficiency mice weighing more than 20 grams, male or female. After purchasing, they were housed in SPF cages for several days.

[0051] 3. Tail vein injection, from the tail vein injection ≥ 10 6 Cancer cells were housed in SPF grade cages.

[0052] 4. Weighing. Weighing every week after three weeks of injection. If the body weight is less than 16 grams, it must be closely observed to prevent death.

[0053] 5. Imaging One month later, an isotope bone scan was performed every two weeks, and bone metastases were found and removed for in vitro culture.

[0054] 6. In vitro subculture of bone metastatic cells Repeat the above work until a new subpopulation of bone metastatic cells is obtained.

[0055] The results showed that radioactive radioactivity in the right upper jaw, left humer...

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Abstract

Adenocarcinoma of the lung cell strain with bone transfer high potency and its construction are disclosed. The process is carried out by using human lung adenocarcinoma SPC-A-1 cell strain as source cell, immunizing deficiency mouse in vivo, externally continuously screening, image detecting from nuclein bone and constructing adenocarcinoma of the lung cell strain SPC-A-1 BM with bone transfer high potency. Its advantages include less cell volume, fast cell growth speed, better infiltration and transfer ability. It can be used for lung cancer transfer.

Description

technical field [0001] The invention belongs to the fields of biotechnology and microbial animal cell lines, and in particular relates to a lung adenocarcinoma cell line with high potential for bone metastasis and its establishment method and application. Background technique [0002] Lung cancer is one of the most common and most malignant tumors in clinical practice, and ranks among the top three in the incidence and mortality of malignant tumors in the world. In my country, especially large and medium-sized cities, lung cancer has always been the first malignant tumor killer since the 1980s. At present, the principle of clinical treatment of lung cancer is still multidisciplinary comprehensive treatment based on surgery. After decades of unremitting efforts, a considerable number of patients have achieved long-term survival by adopting early diagnosis, early treatment and active comprehensive treatment (including surgery, radiotherapy, chemotherapy and biological therapy...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/08
Inventor 扬顺芳
Owner SHANGHAI CHEST HOSPITAL