Mycobacterium Smegmatis preparation and use thereof

Inactive Publication Date: 2007-05-30
NAT INST FOR THE CONTROL OF PHARMA & BIOLOGICAL PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

First, HBV infects the precursor cells of DCs (dendritic cells) or mature DCs and proliferates in them, impairing the function of DCs [1.Wang FS, Xing LH, Liu MX, Zhu CL, et al.Dysfunction of peripheral blood dendritic cells from patients wiTh chronic hepatitis B virus infection. World J Gastroenterol.2001; 7(4):537-41.2. Lohr HF, Pingel S, Bocher WO, et al. Reduced virus specific T helper cell induction by autologous dendritic cells wi in patients chronic hepatitis B-restoration by exogenous interleukin-12.Clin Exp Immunol.2002;130(1):107-14.3.Beckebaum S, Cicinnati VR, Zhang X, et al.Hepatitis B virus-induced defect ofmonocyte-derived dendritic cells leads to impaired T helper type 1 response in vitro: mechanisms for viral immune escape.Immunology.2003;109(4):487-95.], which reduces the ability to secrete IL-12 and limits the differentiation of Th0 cells into Th1 cells

Method used

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  • Mycobacterium Smegmatis preparation and use thereof
  • Mycobacterium Smegmatis preparation and use thereof
  • Mycobacterium Smegmatis preparation and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1: the preparation of Mycobacterium smegmatis preparation

[0046] M. smegmatis preparations can be prepared using different physical lysis methods, most preferably high pressure disruption. The selected bacterial strain is Mycobacterium smegmatis (Mycobacterium smegmatis), and the present embodiment selects the Mycobacterium smegmatis of bacterial strain number CGMCC NO.0795 as an example to illustrate, and the CGMCC NO.0795 bacterial strain is recorded in the published prior application CN1483812A . Other M. smegmatis strains are also suitable.

[0047] 1. Preparation of Mycobacterium smegmatis preparation by high pressure crushing method

[0048] Take freshly cultured Mycobacterium smegmatis on modified Roche’s medium, elute with normal saline to collect the bacteria, centrifuge and wash several times with normal saline, weigh accurately, and dilute with lyophilized protection solution (phosphate buffer) to prepare The bacterium suspension that becomes ...

Embodiment 2

[0074] Example 2: Effects of Mycobacterium smegmatis preparations on delayed-type hypersensitivity and T lymphocyte proliferation in mice

[0075] T lymphocytes are important regulatory cells and effector cells of the body's immune response. Delayed hypersensitivity and T lymphocyte proliferation are used as the basic parameters of in vivo and extracellular immune functions to evaluate the body's immune function. This example illustrates the effects of M. smegmatis preparations on delayed-type hypersensitivity and T lymphocyte proliferation in mice.

[0076] The mice in the experimental group and the mice in the control group were subcutaneously injected with 0.25 mg of Mycobacterium smegmatis preparation and an equal volume of normal saline, once every two weeks, for a total of two injections. Two weeks after the last immunization, the right footpad was challenged with 10μgPPD / 20μl, while BSA was used as a control, and the same dose was injected into the left footpad of the m...

Embodiment 3

[0085] Example 3: Effects of Mycobacterium smegmatis preparations on the production of Th1 and Th2 cytokines in mice

[0086]Th cells are important regulatory cells and effector cells of the immune system. According to the different cytokines secreted, Th cells are divided into two categories: Th1 and Th2. The former mainly secrete IFN-γ and IL-2, etc., and the latter mainly secrete IL- 4. IL-5 and IL-10, etc. The dynamic balance of Th1 / Th2 is regulated by many factors, among which IL-12 is considered as the initiating factor of Th1 response, which promotes the differentiation of Th0 cells into Th1 cells. Th1 / Th2 response maintains a dynamic balance, which is crucial to the immune status of the body. It has been found that various diseases of the respiratory system are related to Th1 / Th2 imbalance. For tuberculosis, Th1 dominant response mediates protective immune response, while Th2 dominant response mediates delayed allergic reactions. Accompanied by pathological immune da...

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Abstract

The invention provides a mycobacterium Smegmatis preparation, which is prepared from Mycobacterium Smegmatis through fragmenting decomposition, such as heavy pressure fragmenting method or ultrasonication method. The Mycobacterium Smegmatis is a safe and effective immuno-modulatory agent for enhancing normal body immune function, accelerating the restoration of the low body immune functions and inhibiting over-strong immune response of the immune sthenic body, i.e. bidirectional immunological control response.

Description

technical field [0001] This application relates to the field of biotechnology, immunology and the field of pharmaceutical use of microbial products, and specifically relates to the verification of immune regulation function of Mycobacterium smegmatis preparations and its use in the prevention and treatment of tuberculosis, asthma, hepatitis B and other diseases. Background technique [0002] Tuberculosis is one of the top ten diseases that endanger human health announced by the World Health Organization (WHO). According to WHO estimates, nearly 1 / 3 of the world's people have been infected with Mycobacterium tuberculosis, and there are 20 million active tuberculosis patients, an increase of 800 every year. 10,000 to 10 million patients and 3 million deaths from TB; if control strategies are not further improved, it is estimated that between 2000 and 2020, 1 billion people will be infected with TB, 200 million people will suffer from TB disease, and 35 million people will die ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/34A61K35/74A61K39/02A61P31/06A61P37/02A61P11/06A61P1/16A61K35/745
Inventor 王国治徐苗陈保文沈小兵苏城
Owner NAT INST FOR THE CONTROL OF PHARMA & BIOLOGICAL PROD
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