Electrochemical biosensor test strip, fabrication method thereof and electrochemical biosensor

Abstract

Electrochemical biosensor test strip, fabrication method thereof and electrochemical biosensor are disclosed. The electrochemical biosensor test strip is fabricated by cutting a groove in a first insulation base in the breadth direction, forming two electrodes parallel to length direction on the first insulation base by sputtering using shadow mask, fixing a reaction material comprising an enzyme which reacts an analyte and generates current corresponding to the concentration of analyte across the two electrodes on the groove of the insulation base, and affixing a cover to the first insulation base. The groove of the first insulation base and the cover make a capillary at the position where the reaction material is fixed. The fabrication method can lower the cost for fabricating the test strip by forming thin electrodes.

Description

technical field [0001] The present invention relates to a test strip for an electrochemical biosensor for quantitative analysis of an analyte of interest, a method of manufacture thereof, and an electrochemical biosensor using the test strip. Background technique [0002] In the medical field, electrochemical biosensors are widely used to analyze biological substances including blood. Among such biosensors, electrochemical biosensors utilizing enzymes are the most predominant biosensors in hospital or clinic laboratories because of their ease of use and high measurement sensitivity, which allows for rapid acquisition of test results. For electrochemical biosensors, an electrode method has recently been widely adopted. For example, in electrode systems fabricated by screen printing, analytes of interest can be quantitatively measured by immobilizing reagents including enzymes on the electrodes, adding a sample, and then applying a voltage across the elec...

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Problems solved by technology

[0009] However, the existing commercially available electrochemical biosensor test strips, including the biosensor test strip described in the above-mentioned patent, have the following serious problems: because the electrodes are all fabricated on the plane of the substrate , and the reagents including enzymes are immobilized on the electrode, and during the immobilization process, the reagents in the liquid phase tend to flow down, so the reagents are difficult to immobilize in some forms

In addition, bonding thick wires to the plastic film will reduce the detection efficiency of the test strip of the electrochemical biosensor

Furthermore, between the thick wire and the plastic film, due to the weak bonding strength, it is easy to disengage; and the cost of the thick electrode material is high

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