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Microbially resistant compositions

A technology of antimicrobial properties and composition, which is applied in the fields of biocides, food science, antibacterial drugs, etc., and can solve the problems that no antimicrobial agents are widely accepted

Inactive Publication Date: 2002-05-22
UNIVERSITY OF OTAGO +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it has not seen widespread acceptance as an antimicrobial agent due to the required concentrations and combined effects on the organoleptic quality of processed foods

Method used

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  • Microbially resistant compositions
  • Microbially resistant compositions
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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0093] Part A

[0094] raw material

[0095] Strains, Media and Chemicals

[0096] Listeria monocytogenes strain L45 and Staphylococcus aureus strain R37 were obtained from the strain culture collection of Dr Roger Cook, New Zealand Meat Processing Research Institute, and Escherichia coli 0157:57 strain NCTC 12900 was obtained from Heather Brooks, Department of Microbiology, University of Otago Dr.'s strain culture collection. Stock solutions of all strains were stored in skim milk at -70°C and subcultured when needed on Plate Count Agar (PCA) (Difco Laboratories, Detroit, Michigan, USA) or on Blood Agar (BA ) (Columbia Agar Base (GIBCO BRL, Life Tech Ltd, Paisly, UK) supplemented with 5% human whole blood (Dunedin Public Hospital, Dunedin, New Zealand)) for subculture. In normal use, the strains were kept in plate culture and subcultured every two weeks. All commercial media were prepared according to the manufacturer's instructions. Monolaurin (1-monolauryl-rac-glycerid...

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Abstract

Peroxidase systems such as the Lactoperoxidase system (LPS) are combined with antimicrobial fatty acids or fatty acid derivatives to produce a synergistic antimicrobial composition. These compositions are disclosed as being useful for rendering surfaces, and also products such as food or cosmetics, resistant to microbes. A preferred form of the composition contains lactoperoxidase, monolaurin or sodium dodecyl sulphate, a source of peroxide, and thiocyanate ions.

Description

technical field [0001] The present invention relates to antimicrobial compositions and methods for their preparation. It also relates to methods of use of these compositions, as well as products rendered antimicrobial due to the addition or application of these compositions. Background technique [0002] Lactoperoxidase (LP) is an enzyme that is part of the natural non-immune defense system in milk and mucous secretions such as saliva, tears and small intestinal secretions. LP and various naturally occurring cofactors form the lactoperoxidase system (LPS) with antimicrobial activity. [0003] LPS consists of LP (derived from milk), a source of peroxide, and a cofactor (usually thiocyanate). In many cases, glucose oxidase (from the microorganism) and glucose are added to provide a source of hydrogen peroxide. The enzyme system employed is often preferred due to the need to ensure sustained delivery of peroxide. But this requires aerobic conditions, so a direct source of p...

Claims

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Application Information

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IPC IPC(8): A01N37/12A01N63/50A23K3/00A23L3/3463A23L3/3508A23L3/3526A23L3/3535A23L3/3571A23L3/358A61K8/00A61K8/19A61K8/20A61K8/22A61K8/23A61K8/30A61K8/36A61K8/37A61K8/46A61K8/66A61K8/72A61P31/04A61Q11/00A61Q17/00A61Q90/00
CPCA61K8/23A01N63/02A23L3/3508A61K8/361A61K8/22A23L3/3463A61Q11/00A61K2800/51A23L3/3535A61K8/20A61K8/463A61K8/66A61K2800/59A23L3/3526A61Q17/005A23K3/00A23L3/358A61K2800/52A61K8/375A23K30/00A61P31/04A01N63/50A61K2800/882A01N59/24A01N59/00A01N41/02A01N37/12A01N37/06A01N37/02A01N63/10A01N2300/00
Inventor P·F·芬尼塞R·S·西蒙兹
Owner UNIVERSITY OF OTAGO
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