The invention discloses a fluorescence probe for detecting bisulfate and a preparation method and application of the fluorescence probe for detecting the bisulfate. The molecular formula of the probeis C52H42N4O6, and the structural formula of the probe is as shown in the following formula. The fluorescence probe I prepared by the invention is simple and convenient in synthesis, high in yield andeasy to prepare, and suitable for amplification synthesis and practical production application. The fluorescence probe can use an ultraviolet-visible method and a fluorescence method to detect bisulfite ions, is good in selectivity, fast in detection, and suitable for naked eye detection, and not interfered by anions of acetate ions, thiocyanate ions, nitrite ions, nitrate ions, hydrogensulfate ions, carbonate ions, fluoride ions, chloride ions, bromide ions, iodide ions, cysteine, homocysteine, glutathione and biological mercaptan, can be applied to the field of ion detection as a new probeand has broad application prospects.