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Method for detecting thiocyanate ion based on fluorescence resonance energy transfer

A technology of fluorescence resonance energy and thiocyanate, which is applied in the field of analysis, can solve the problems of time-consuming, high detection cost, and inability to be widely used, and achieve high-sensitivity and sensitive detection effects

Inactive Publication Date: 2015-01-28
NANCHANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although these methods have high sensitivity, there are many pre-treatment steps for samples, which are relatively time-consuming, high detection costs, and require professional operations, making them not widely used.
At present, my country has not released the standard for the detection method of thiocyanate in milk, therefore, it is necessary to establish a fast, simple and sensitive method for the detection of thiocyanate in milk samples

Method used

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  • Method for detecting thiocyanate ion based on fluorescence resonance energy transfer
  • Method for detecting thiocyanate ion based on fluorescence resonance energy transfer
  • Method for detecting thiocyanate ion based on fluorescence resonance energy transfer

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Embodiment 1

[0022] A method for detecting thiocyanate ions based on fluorescence resonance energy transfer, the specific steps are as follows:

[0023] (1) Preparation of gold nanosolution glue:

[0024] Wash all the glassware, soak it with freshly prepared aqua regia, wash it with ultrapure water and dry it, and prepare the gold nanosolution glue by trisodium citrate reduction method: add 1 mmol / L chloroauric acid to a 250 mL three-necked flask 100 mL of aqueous solution was heated to boiling under full stirring, and 38.8 mmol / L trisodium citrate aqueous solution was quickly added under stirring, so that the golden yellow chloroauric acid aqueous solution turned wine red within 2 minutes, continued to condense and reflux for 15 minutes, and cooled naturally to At room temperature, the gold nano-sol was prepared, and stored at 4°C for subsequent use;

[0025] (2) Synthesis of fluorescein-gold nanocomposites:

[0026] Put 30 μL at a concentration of 1.0 x 10 -5 mol of fluorescein was ad...

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Abstract

The invention discloses a method for detecting thiocyanate ion in milk based on fluorescence resonance energy transfer. The method comprises the following steps: preparing gold nano solution gel; synthesizing a fluorescein-gold nano complex; detecting the thiocyanate ion and detecting the actual sample. According to the method, the thiocyanate ion is quantitatively detected through fluorescence recovery of a gold nano-fluorescein system caused by competitive binding of the thiocyanate and fluorescein on the surface of the gold nano, the total detection time used by the provided detection method is less than 30 minutes, the thiocyanate ion in the milk can be simply, rapidly and sensitively detected, the method has extremely high sensitivity, the detection limit is 0.09nM, and convenience is provided for research, production and supervision in the future.

Description

technical field [0001] The invention relates to a method for detecting thiocyanate ions based on fluorescence resonance energy transfer, belonging to the technical field of analysis. Background technique [0002] Thiocyanate is a chemical raw material used in various industries such as medical treatment, printing and dyeing. When it exists in sewage, industrial wastewater and pesticide residues, it has a great impact on the environment and human body. Thiocyanate is one of the important components of the lactoperoxidase antibody system. It is often used as a fresh-keeping agent for fresh milk. However, thiocyanate is toxic, and excessive intake can cause acute poisoning; a small amount of intake can inhibit The transfer of iodine causes goiter. On December 12, 2008, the Ministry of Health issued a list of non-edible substances that may be illegally added to food and food additives that are easily abused (the first batch), which clearly stipulates that sodium thiocyanate in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 吴芳英宋娟万益群
Owner NANCHANG UNIV
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