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Method of extracting peucedanin from angelica

A technology for imperatorin and angelica, applied in the field of extracting imperatorin, can solve the problems such as purity difficult to meet the requirements of reference substances, low extraction efficiency, easy explosion, etc. toxic side effects

Inactive Publication Date: 2003-07-02
深圳新协合健康管理有限公司
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Problems solved by technology

[0003] In recent years, the demand for imperatorin, the reference substance, in the quality analysis of the traditional Chinese medicine Angelica dahurica and Chinese patent medicines has been increasing. The methods for separating imperatorin recorded in the literature use ether as the extraction solvent, and the boiling point of ether is Low, easy to explode; some extract by percolation and cold soaking, but the extraction efficiency of these two methods is low and time-consuming; some are refined by recrystallization, but the purity after recrystallization is difficult to meet the requirements of the reference substance

Method used

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Embodiment Construction

[0007] 1. Take 5 kg of the crushed Angelica dahurica medicinal material, extract twice with 95% ethanol (add 8 times the amount for the first time, add 6 times the amount for the second time), decoct for 3 hours each time, filter with four layers of gauze to obtain the filtrate. Recover the ethanol in the filtrate to no alcohol, dilute with 1000mL water, extract 5 times with 3000mL chloroform, and combine the chloroform layers. The chloroform in the chloroform layer was evaporated to dryness, and vacuum-dried to anhydrous to obtain 100 g of extract. The extract is mixed with 200g of silica gel, and eluted with petroleum ether-acetone on the chromatographic silica gel column (the silica gel on the upper column is 1500g), and the elution ratios are 100:0, 100:1, 100:2, 100:4 , 100:8, 10 retention volumes were eluted in each ratio, and the crude imperatorin was obtained in the 100:2 eluted part. 2 g of the reference substance of imperatorin was purified on the preparative liquid...

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Abstract

A process for extracting ammidin from dahurian angelica root includes such steps as breaking to obtain coarse powder, extracting in alcohol, decocting, filtering, recovering alcohol from the filtrate, diluting with water, extracting with chloroform for 3-5 times, collecting the liquid extract, volatilizing chloroform, vacuum drying, to obtain extract chromatography, eluting with petroleum ether-acetone, and refining by reverse high-efficiency liquid-phase chromatography. Its advantages are high purity up to 98% or more, and good technological stability.

Description

technical field [0001] The invention relates to a method for purifying traditional Chinese medicine, in particular to a method for extracting imperatorin from Angelica dahurica. Background technique [0002] Drug reference substances in my country refer to those recorded in the Chinese Pharmacopoeia and ministerial standards, which are used to obtain the accuracy and consistency of identification, content determination, impurity and related substance inspection and other items during drug testing, and are used to compare with the tested samples to determine their quality. A physical standard of quality. With the development of modern science and technology, in the field of drug analysis, the application range of traditional Chinese medicine reference substances is constantly expanding, and the demand for their quantity and variety is also increasing. It is often the appearance of a reference substance, which drives the establishment of a batch of drug testing methods and qua...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D493/04
Inventor 毕开顺陈巨余李清张林疆侯晓红于滨
Owner 深圳新协合健康管理有限公司