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Magnetic separation type immunological reaction detection device of using fluorescence imaging and detection method

A technology of fluorescence imaging and immune reaction, which is applied in the directions of measurement devices, fluorescence/phosphorescence, and analytical materials, can solve the problems of high cost, poor flexibility, and complicated fluorescent labeling process, and achieve high cost-effectiveness, simple structure, and Conducive to the effect of popularization and application

Inactive Publication Date: 2003-07-23
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although this method simplifies the B-F separation process, it still requires a complicated fluorescent labeling process, and since the antibody is fixed on the surface of the total reflection mirror, the total reflection mirror must be replaced after each immune reaction, and the use cost High, poor flexibility

Method used

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  • Magnetic separation type immunological reaction detection device of using fluorescence imaging and detection method
  • Magnetic separation type immunological reaction detection device of using fluorescence imaging and detection method
  • Magnetic separation type immunological reaction detection device of using fluorescence imaging and detection method

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Embodiment Construction

[0020] The technical solutions of the present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0021] Such as figure 1 As shown, the basic structure of the detection device of the present invention consists of a reaction tank 1, an optical filter 4 and a magnetic pole 5, and the optical filter 4, magnetic pole 5 and imaging objective lens 6 are sequentially placed under the reaction tank 1. A liquid inlet 2 is opened on the upper part of the baffle on one side of the reaction tank 1 , and a liquid outlet 3 is opened on the lower part of the baffle on the other side of the reaction tank 1 .

[0022] In one embodiment of the present invention, first press figure 1 The structure diagram shown constitutes the system, and the detection process is as follows:

[0023] 1. Coat antibodies (such as mouse immunoglobulin G) on magnetic particles to form a solution, and immobilize

[0024] volume of mixed solution is dropped i...

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Abstract

The detection device is composed of the immune reaction tank and the magnetic pole. The magnetic particles of coating antibody are used as the reaction substrate. Specificity fluorescein-labelled antibody is utilized to display antigen to form antibodies-antigens-fluorescein-labelled antibody resultant of reaction. Since the action of the magnetic pole, the immune reaction resultant in combination with magnetic particles is adsorbed at the bottom of the immune reaction pond. The limited solution makes the antigen tested distribute on the bottom of the reaction tank. The fluorescence image of the reaction resultant is obtained by using laser source to irradiate the reaction tank. The invention raises the detection sensitivity, realizes real time, sensitive, accurate and multiform immune reaction detection.

Description

Technical field: [0001] The invention relates to a magnetic separation type fluorescent imaging immune reaction detection device and detection method, which is a method of absorbing antibodies on the surface of magnetic particles to carry out antigen-antibody immune reaction, and then using the specific fluorescent antibody of the antigen to bind to the surface of magnetic particles The antigen binding on the antibody realizes the qualitative and quantitative optical detection device of the fluorescent imaging detection of the immune response, and uses this new type of detection device to detect the immune response. Background technique: [0002] Immunofluorescence detection technology is widely used in many aspects of biology and medicine such as immunology, microbiology, cell and histology, pathology, oncology and clinical laboratory science. Immunofluorescence detection technology has the characteristics of high specificity, strong sensitivity and wide application range. ...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N33/53G01N33/533G01N33/537
Inventor 赵海鹰于常青窦晓鸣黄梅珍殷光中袁波
Owner SHANGHAI JIAO TONG UNIV