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Nervous duct and membrane with fixed nerve regeneration promoting agent with surface modified by plasma and their preparations

A nerve regeneration and plasma technology, applied in catheters, coatings, etc., can solve problems such as adverse reactions of the body, affecting nerve growth promoters, and the diffusion of nerve growth promoters.

Inactive Publication Date: 2003-09-03
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the application of the above-mentioned nerve conduits, most of these nerve regeneration accelerators are introduced into the inside of the conduit or on the surface of the conduit material by physical adsorption. Since the tube wall of the nerve conduit must be a semi-permeable system, it is beneficial for the nutrients in the body fluid to enter the conduit. The discharge of metabolites, therefore, under the action of body fluids, these physically adsorbed nerve growth promoters are easy to flow out early through the tube wall, causing the diffusion of nerve growth promoters in the body, affecting the concentration of nerve growth promoters in the tube to play a role , and may cause adverse reactions in the body

Method used

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  • Nervous duct and membrane with fixed nerve regeneration promoting agent with surface modified by plasma and their preparations
  • Nervous duct and membrane with fixed nerve regeneration promoting agent with surface modified by plasma and their preparations
  • Nervous duct and membrane with fixed nerve regeneration promoting agent with surface modified by plasma and their preparations

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The preparation of embodiment 1 lysine plasma surface modification coating film

[0037] PGLA with a molecular weight of 200,000 is melted on the glass sheet and cooled into a uniform transparent film. Put it into a 13.56MHz glass plasma reactor with a diameter of 10cm and a length of 55cm. The electrodes are capacitively coupled. Vacuum to vacuum degree 3.5×10 -2 pa, pass through the prepared lysine aqueous solution with a concentration of 30%, and introduce the reactor through argon gas. Adjust the flow meter to control the gas flow to 100sccm, the vacuum degree to 50Pa, adjust the pulse ratio to 80%, the "on" time interval to 3ms, the power to 40w, turn on the power supply and discharge polymerization for 3hrs to obtain polylysine-coated PGLA film, lysine Infrared spectra of acid coatings such as figure 1 As shown, the amino acid amide I (1620cm -1 at C=O stretching vibration peak), amide II (1520cm -1 N-H deformation vibration peak), amide III gene (1351cm -1 ...

Embodiment 2

[0038] Example 2 Preparation of vinyl acetic acid plasma surface modification coating film

[0039] PGLA with a molecular weight of 200,000 is melted on the glass sheet and cooled into a uniform transparent film. Put it into a 13.56MHz glass plasma reactor with a diameter of 10cm and a length of 55cm. The electrodes are capacitively coupled. Vacuum to vacuum degree 3.5×10 -2 pa, into the vinyl acetic acid solution, through the argon into the reactor. Adjust the flow meter to control the gas flow to 30sccm, the vacuum range to 120Pa, the power to 20w, turn on the power supply for discharge polymerization for 2 hours, adjust the pulse ratio to 100-1%, and the time interval of "on" to be 10ms to obtain the polyvinyl acetic acid modified coating PGLA membrane. XPS spectrum of vinyl acetic acid coating as figure 2 Shown, C in C-C, C-O, C=O functional groups 1s The atomic binding energies are 284.6eV, 286.0eV, and 287.6eV, respectively. From figure 2 It can be seen that whe...

Embodiment 3

[0040] Example 3 Immobilization reaction of nerve regeneration promoter CNTF on membrane

[0041] The PGLA membrane modified with lysine plasma coating prepared in Example 1 was sterilized with ethylene oxide at room temperature, and the carbodiimide biocatalyst was formulated into a 20% PBS solution. Nerve regeneration-promoting factor CNTF (ciliary neurotrophic factor) was made into PBS aqueous solution with a concentration of 50ng / ml. The PGLA membrane was placed in the carbodiimide solution for 30 minutes, rinsed with sterile distilled water after taking it out, and then placed in the CNTF solution with a volume ratio of the solution to the material to be treated at 2:1, and reacted at 4°C for 32 hours. After taking it out, rinse it three times with PBS solution for later use. The concentration of CNTF immobilized on the membrane measured by Elisa method was 13ng / cm 2 .

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Abstract

A nerve catheter or membrane for regenerating or repairing nerve features that a plasma surface modification method is used to immobilize the nerve regeneration promoter on its surface and the fibres, non-woven fabric, or sponge filler which are coaxially inserted in said catheter to induce the nerve cells to grow along surface, resulting in higher effect.

Description

technical field [0001] The invention relates to a nerve conduit or membrane of a plasma surface modified and immobilized nerve regeneration promoting agent and a preparation method thereof. The nerve conduit can be implanted in a living body to repair defective nerves, and the membrane material can be used for culturing nerve cells in vitro. Background technique [0002] The nervous system is the most important organ of the human body, which controls the sensory and motor functions of the human body. Once the nerve is damaged, it often means the loss of sensory and motor functions in some parts of the human body, causing great pain to the patient. But so far, the repair of defective nerves and the reconstruction of functions are still the focus and difficult problems of human beings. [0003] At present, the clinical repair of defective nerves mainly adopts autologous or allogeneic nerve transplantation; anastomosis of adventitia and perineurium; Autologous nerve transplan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L29/04A61L29/08
Inventor 张菁谢涵坤胡群华陈皓
Owner DONGHUA UNIV
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