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Solid fermentation process of streptomyces flavus and its production equipment

A technology of Streptomyces flavus and solid fermentation, which is applied in the directions of bacteria, botanical equipment and methods, biocides, etc., can solve the problems of small quantity of Streptomyces flavus, difficulty in disinfection, no production process and production equipment, etc. Achieve the effect of high sporulation rate and effective viable bacteria ratio and less bacterial contamination

Inactive Publication Date: 2003-09-17
青岛地恩地生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the amount of Streptomyces flavinus growing naturally in soil is too small to be insignificant
At present, there is no artificial production of such strains in China, and there is no corresponding production process and production equipment.
[0003] Compared with liquid fermentation, solid fermentation is more difficult to sterilize, the process is more difficult to control, it is easy to contaminate bacteria or mold, and the technology is very difficult, so it is difficult to succeed
Therefore, solid fermentation is rarely used in China, and there are almost no relatively mature solid fermentation processes.

Method used

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  • Solid fermentation process of streptomyces flavus and its production equipment
  • Solid fermentation process of streptomyces flavus and its production equipment
  • Solid fermentation process of streptomyces flavus and its production equipment

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Embodiment 1. a kind of solid fermentation technique of Streptomyces flavinus, it may at least comprise the following steps:

[0031] (1) Mixing of raw and auxiliary materials: the raw and auxiliary materials are composed of 10% bean cake powder, 20% peanut shells, 6% corn flour, 4% millet flour, 8% bran, 50% bran by mass ratio (the same below). % pond mud, 2% lime and the rest of the water, stir evenly, put into the stainless steel sterilization tray, then put the stainless steel sterilization tray into the sterilization cart, push the sterilization cart into the autoclave and close the door closure;

[0032] (2) Autoclaving: Pass the autoclave into superheated steam to heat up and sterilize the raw and auxiliary materials, the pressure is 2 kg / cm2, the temperature is 132°C, and the time is 80 minutes;

[0033] (3) Inoculation: when the raw and auxiliary materials after autoclaving are cooled to 26°C, inoculate the seeds of Streptomyces luteus obtained by liquid ferme...

Embodiment 2

[0042] Embodiment 2. a kind of solid fermentation process of Streptomyces flavinus, it at least comprises the following steps:

[0043] (1) Mixing of raw and auxiliary materials: the raw and auxiliary materials are composed of 20% bean cake powder, 25% peanut shells, 12% corn flour, 2% millet flour, 5% bran, 60 % pond mud, 1% lime and the rest of the water, stir evenly, put into the stainless steel sterilization tray, then put the stainless steel sterilization tray into the sterilization cart, push the sterilization cart into the autoclave and close the door closure;

[0044] (2) Autoclaving: Pass the autoclave into superheated steam to heat up and sterilize the raw and auxiliary materials, the pressure is 3 kg / cm2, the temperature is 128°C, and the time is 60 minutes;

[0045] (3) Inoculation: when the raw and auxiliary materials after autoclaving are cooled to 30°C, inoculate the seeds of Streptomyces luteus obtained by liquid fermentation according to 8% of the mass ratio ...

Embodiment 3

[0053] Embodiment 3. a kind of solid fermentation process of Streptomyces flavinus, it at least comprises the following steps:

[0054] (1) Mixing of raw and auxiliary materials: the raw and auxiliary materials are composed of 16% bean cake powder, 15% peanut shells, 5% corn flour, 8% millet flour, 12% bran, 30% bran by mass ratio (the same below). % pond mud, 5% lime and the rest of the water, stir evenly, put into the stainless steel sterilization tray, then put the stainless steel sterilization tray into the sterilization cart, push the sterilization cart into the autoclave and close the door closure;

[0055] (2) Autoclaving: Pass the autoclave into superheated steam to heat up the raw and auxiliary materials to sterilize, the pressure is 1.5 kg / cm2, the temperature is 135°C, and the time is 90 minutes;

[0056] (3) Inoculation: when the raw and auxiliary materials after autoclaving are cooled to 28°C, inoculate the seeds of Streptomyces luteus obtained by liquid fermenta...

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Abstract

A solid fermenting process of xanthostreptomycete includes such steps as proportionally mixing soybean cake powder, peanut shell, corn flour, millet flour, wheat bran, and lime and water, high-pressure sterilizing at 128-135 deg.C for 60-90 min, inoculating seeds, and fermenting for 144 hr. Its apparatus is composed of dual-screw stirrer, disinfecting stainless steel tray, sterilizing carriage, high-pressure sterilizing pot, collectnig hopper, belt conveyer, humidify meter, fermenting tray and fermenting case.

Description

technical field [0001] The invention belongs to the technical field of Streptomyces luteus fermentation, and more specifically relates to the improvement of a solid fermentation process of Streptomyces luteus and production equipment thereof. Background technique [0002] Existing Streptomyces flavinus multiplies in the soil. During the growth process, it can dissociate a large amount of nitrogen, phosphorus, and potassium that have been solidified in the soil and cannot be absorbed by crops, improve the utilization rate of chemical fertilizers, and effectively alleviate the negative effects of soil compaction caused by extensive use of chemical fertilizers. At the same time, it can generate a variety of growth hormones to promote the growth of crops and improve their resistance to stress. Streptomyces flavinus can also produce a variety of antibiotics to inhibit the growth of pathogens, which is very beneficial to the elimination of soil-borne pathog...

Claims

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Application Information

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IPC IPC(8): A01N63/00C05F11/08C12N1/20
Inventor 张永升徐树建
Owner 青岛地恩地生物科技有限公司
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