Method of extracting oligo proanthocyanidin of grape seed

A technology of oligomeric proanthocyanidins and extraction method, which is applied in the field of grape seed oligomeric proanthocyanidin extraction, and can solve problems such as yield reduction, emulsification, and cumbersome post-processing steps

Inactive Publication Date: 2003-11-12
XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] These methods take a long time to extract, or require several days of long-term soaking, the extraction rate is low, and the post-processing steps are cumbersome. Most of the methods involve ethyl acetate extraction procedures, which are prone to emulsification during the operation process, resulting in a decline in yield

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] a. Grape seeds are crushed by mechanical pulverization, passed through a 10-20 mesh coarse sieve, and degreased with sherwood oil to obtain degreased grape seed pomace and 15% light yellow grape seed oil;

[0021] b. Add 1,000 grams of defatted grape seed pomace to 5,000 milliliters of water-saturated ethyl acetate containing 8.5% phosphoric acid, stir at room temperature for 5 hours, filter, and extract twice at an extraction temperature of 10° C. and an extraction time of 1 hour;

[0022] c. After combining the solutions extracted several times, add 21 grams of calcium hydroxide to neutralize under stirring, stir for 50 minutes, the pH is 5, filter, keep the filtrate, concentrate under reduced pressure, and obtain the crude proanthocyanidin extract;

[0023] d. Dissolve the crude extract with ethyl acetate organic solvent, slowly add 4 times the amount of chloroform under stirring, leave it for 10 hours, filter and dry to obtain 24.6 grams of refined proanthocyanidins,...

Embodiment 2

[0025] a. Grape seeds are crushed by mechanical pulverization, passed through a 10-20 mesh coarse sieve, and degreased with sherwood oil to obtain degreased grape seed pomace and 15% light yellow grape seed oil;

[0026] b. Add 800 grams of defatted grape seed pomace to 2000 milliliters of water-saturated ethyl acetate containing 1% phosphoric acid, microwave bath at 20 ° C, shake for 30 minutes, filter, and extract 3 times;

[0027] c. After combining the solutions extracted several times, add 1.5 g of calcium oxide to neutralize under stirring, stir for 50 minutes, the pH is 7, filter, keep the filtrate, concentrate under reduced pressure, and obtain the crude proanthocyanidin extract;

[0028] d. Dissolve the crude extract with ethyl acetate organic solvent, slowly add 5 times the amount of petroleum ether under stirring, leave it for 5 hours, filter and dry to obtain 18.1 grams of refined proanthocyanidins, the content of proanthocyanidins is 62.2%, and the total phenol con...

Embodiment 3

[0030] a. Grape seeds are crushed by mechanical pulverization, passed through a 10-20 mesh coarse sieve, and degreased with sherwood oil to obtain degreased grape seed pomace and 15% light yellow grape seed oil;

[0031] b. Add 1000 grams of degreased grape seed pomace to 5000 milliliters of water-saturated ethyl acetate containing 0.1% phosphoric acid, extract 2 times, the extraction temperature is 30 ° C, the extraction time is 5 hours, and filter;

[0032] c. After combining the extracted solutions several times, add 0.5 g of calcium hydroxide to neutralize under stirring, stir for 10 minutes, the pH is 5, filter, keep the filtrate, concentrate under reduced pressure, and obtain the crude proanthocyanidin extract;

[0033] d. Dissolve the crude extract with ethyl acetate organic solvent, slowly add 2 times the amount of chloroform under stirring, leave it for 5 hours, filter and dry to obtain 19.6 grams of refined proanthocyanidins, the content of proanthocyanidins is 60.9%,...

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Abstract

The method for extracting grape-seed oligoproanthocyandin includes the following steps: pulverizing grape-seed, sieving and defatting to obtain grape-seed dregs, extracting the grape-seed dregs by using organic solvent containing inorganic mineral acid, feltering, neutralizing with inorganic alkali, filtering and reduced pressure concentrating to obtain proanthocyanidin crude extract, then adding petroelum ether or chlorform into the crude extract, standing still, filtering and drying to obtain refined proanthocyanidin. The utilization of said method can quickly and simple extract the grape-seeds-waste material of winery and can obtain the invented oligoproanthocyanidin whose content is greater than 60% and yield can be up to 2%.

Description

technical field [0001] The invention relates to a method for extracting oligomeric proanthocyanidins from grape seeds, in particular, the oligomeric proanthocyanidins can be obtained by extracting oligomeric proanthocyanidins from grape seeds left over from wineries Background technique [0002] Oligomeric proanthocyanidins are a class of polyphenolic polymers that widely exist in the plant kingdom. They are polymerized with different numbers of flavan-3-ols and flavan-3,4-ols. The constituent units are catechins and cousins. Tea element monomer. Oligomeric proanthocyanidins mainly exist in grape seeds, but also in the winemaking process, and affect the flavor and color of wine. [0003] In addition to playing an important role in the brewing process and plant food, oligomeric proanthocyanidins also have many beneficial effects on human health. Oligomeric proanthocyanidins are powerful natural antioxidants that can remove active oxygen free radicals i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/62
Inventor 赵文军吴雪萍王旭
Owner XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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