Testing method for skin inflammation or irritation and kit

A kit, skin technology, applied in the field of skin inflammation or irritation detection and kit

Inactive Publication Date: 2003-12-17
JOHNSON & JOHNSON CONSUMER COPANIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

One of the potential problems that may arise when using interleukins or other proteins as markers of inflammation is their potential interaction with the skin care products being tested

Method used

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  • Testing method for skin inflammation or irritation and kit
  • Testing method for skin inflammation or irritation and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0094] Example 1 - Method of Measuring the Irritant Effect of Water on the Skin

[0095] As a means of determining the sensitivity of the method for measuring subclinical effects, we selected two bland fluids to determine the inflammatory potential following exposure. Some of the mildest liquids that come to mind are the different types of water that are present during cleansing of the skin, and it is also a major ingredient in many topical skin care products. In this example, we use PGE 2 Inflammatory responses after skin exposure to deionized water and tap water were measured as markers.

[0096] The determination follows the following method:

[0097] 1. Determination of PGE contained in secretions present on the skin surface along the volar forearm of the subject 2 (Cuderm Corporation, Dallas, Texas) by placing a mild adhesive-coated microporous plastic film, Sebutape(R) (Cuderm Corporation, Dallas, Texas) on the skin in direct contact with the skin. After 1 minute the...

Embodiment 2

[0105] Example 2 - Possible Interaction of Anionic Surfactants with Interleukin-α (IL-1α)

[0106] One concern with using IL-la as a marker of inflammation or irritation is its potential interaction with surfactants often included in topical skin care compositions. This example illustrates this interaction by measuring the apparent level of IL-1α expressed on the skin as a function of the concentration of sodium lauryl sulfate (SLS, Stepan Co., Northfield, IL) in the test fluid.

[0107] SLS concentration in deionized water

[0108] Table 2 shows the dose-dependent data for IL-la after exposing the skin to different concentrations of SLS. The amount or response of markers of inflammation is expected to increase with increasing levels of anionic surfactant. This was observed at low SLS levels, but not at the highest SLS concentration detected in the study, raising doubts about the feasibility of the method. We wish not to be bound by theory, and believe that high ...

Embodiment 3

[0109] Example 3 Inflammation caused by mild face wash

[0110] pure water

Glycerin

Dodecyl Glucoside

Decyl Glucoside

Cocoamidopropyl betaine (cocomidopropyl

Betaine)

Cocoamide DEA

Glycereth-7

Ammonium dodecylsulfonate

Sodium cocoyl sarcosinate

Ethylene glycol stearate

PEG-120 Methyl Glucose Dioleate

Tetrasodium EDTA

DMDM hydantoin

citric acid

spices

[0111] Table 4 shows the determination of PGE using the above method 2 The degree of irritation caused by the face wash compared to deionized water as indicated by the amount expressed.

[0112] subjects

Deionized water

facial cleanser

1

1.98

1.81

2

1.48

1.26

3

1.30

0.67

4

0.83

2.11

5

1.49

1.72

6

2.12

3.07

7

1.2...

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PUM

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Abstract

A non-invasive, in vivo method for measuring sub-clinical or clinical inflammation or irritation of mammalian skin from exposure of the skin to a topical skin care product, exposure to an external aggression or combinations thereof is disclosed. In one embodiment, the method includes the steps of collecting eicosanoid from the skin using a non-invasive collection device and analyzing levels of eicosanoid collected from the skin. A kit for measuring a marker of skin irritation or inflammation is also disclosed. The kit includes a non-invasive collection device for collecting secretions from the skin surface, and an immunoassay for measuring level of eicosanoid in the secretions.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of a co-pending provisional application filed September 6, 2001 with serial number 60 / 317638. field of invention [0003] The invention relates to a detection method and kit for skin inflammation or irritation. More specifically, the present invention relates to methods and kits for non-invasive in vivo detection of skin inflammation or irritation. Background of the invention [0004] The skin is the largest organ of the human body, and its appearance has a huge impact on a person's self-confidence and quality of life. In fact, the initial impression of someone (and this impression lasts a long time) is often created by the person's appearance (facial features, hair color, etc.). [0005] Consumers use skin care products to treat their skin conditions (such as acne, psoriasis, fine lines and wrinkles), improve skin health (such as dry, flaky skin; oily skin) or to cover small blemi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50A61B5/00A61B5/107G01N33/48G01N33/53G01N33/68G01N33/88
CPCG01N2800/20G01N33/88Y10T442/2525G01N33/6893G01N33/50
Inventor K·黄N·蒂尔尼B·威甘德
Owner JOHNSON & JOHNSON CONSUMER COPANIES
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