Method for increasing growing and propagating ability of fungus agent in soil

A fungal inoculum and soil technology, which is applied in the field of improving the growth and reproduction capacity of fungal inoculants in soil, can solve the problems of nutrient composition changes and inappropriate fungi, and achieves the effects of low cost, improved field control effect and high efficiency.

Inactive Publication Date: 2004-02-11
YUNNAN UNIV
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Problems solved by technology

This method has the following disadvantages: due to the transformation of the medium components used by microorganisms, the nut

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach ： Embodiment 1

[0023] The specific embodiment: Embodiment 1: Nutrients improve the germination and reproduction of Verticillium chlamydoides in soil

[0024] Nutrition

[0025] The results show that several nutrients can greatly improve the germination and growth of Verticillium verticillium in soil, and the CFU reaches 10 4 -10 6 , while the CFU of the control was only 23. Embodiment 2: Nutrient dilution factor is on improving the impact of Verticillium chlamydogermis germination and reproduction in soil

Embodiment 2

[0026] Nutrients (50% molasses, 25% glucose, 25% sweet potato flour) are diluted from 10 times to 100 times, and the nutrient substances diluted respectively by the bacterial agent of Verticillium chlamydota are mixed in a volume ratio of 1: 10, and then according to

[0027] to dilute

[0028] The results showed that when the nutrient was diluted from 10 times to 100 times, it could still significantly improve the germination and growth of Verticillium chlamydoides in the soil, and the CFU was at 10 5 above. Embodiment 3: The ratio that nutrient substance mixes with bacterial agent, placement time and temperature are on improving the influence of germination and reproduction of Verticillium chlamydoides in soil

Embodiment 3

[0029] mix

[0030] The results show that the mixing ratio of the bacterial agent and the nutrient substance is effective between 1:1-50. Furthermore, there was no apparent effect on enhancing the germination and growth of Verticillium chlamydoides in soil within the time and temperature ranges determined.

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Abstract

The present invention relates to the method of raising the growth and propagation ability of fungus in soil, and belongs to the field of pesticide technology. The nutritive matter of molasses, corn slurry, corn cob, manioc powder, sweet potato powder, soybean powder, crop straw, glucose, cane sugar, starch, palmic acid, starch, citric acid or their mixture in any ratio is diluted to 10-100 times, and mixed with verticillium chlamydosporium in the volume ratio of 1-50, and the mixture is let stand at 18-30 deg.c for 20-50 hr before applied in soil. The present invention can raise the growth and propagation ability and thus plant eelworm preventing and treating effect in field of verticillium chlamydosporium in biological preparation.

Description

Technical field: [0001] The invention relates to a method for improving the growth and reproduction ability of fungal agents in soil, and belongs to the technical field of biological pesticides. Background technique: [0002] "Soil antibacterial effect, fungistasis of soil" refers to the phenomenon that the germination of microbial propagules (including fungi, bacteria, actinomycetes, etc.) in natural soil or in contact with soil is inhibited. Bacteriostasis exists on bacteria, actinomycetes, and fungi, but fungi are the most sensitive, which can keep the propagules of microorganisms in a dormant state for a long time in the soil (Lockwood, 1977). When biocontrol agents are used to control plant diseases and insect pests in the soil or on the soil surface, due to the influence of soil antibacterial effect, the biocontrol bacteria cannot germinate in the soil or on the soil surface or the germination rate is very low, generally the germination rate is low less than 30%, ther...

Claims

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Application Information

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IPC IPC(8): A01N63/04C12N1/14
Inventor 莫明和张克勤周薇
Owner YUNNAN UNIV
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