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Use of pulse-activating injection in preparing synergetic medicine for treating tumor

A technology of Shengmai injection and medicine, which is applied in the field of preparation of synergistic drugs for the treatment of tumors, traditional Chinese medicine Shengmai injection, and preparation of synergistic drugs for the treatment of malignant tumors, which can solve problems such as cross-resistance and achieve the effect of expanding indications

Inactive Publication Date: 2004-02-11
HEHUANG PHARMA SHANGHAI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, the only potent potentiator with definite efficacy is CF (only for 5-Fu potentiation)
Carboplatin is the second-generation platinum anticancer drug, its mechanism of action is similar to that of cisplatin, but it has partial cross-drug resistance with cisplatin

Method used

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  • Use of pulse-activating injection in preparing synergetic medicine for treating tumor
  • Use of pulse-activating injection in preparing synergetic medicine for treating tumor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Synergistic effect test of Shengmai injection on the chemotherapy drug DDP of human colon cancer cell Lovo:

[0045] Cell line culture: cultured with the vigorously proliferating human colon cancer cell Lovo, sucked out the culture solution, washed the cells three times with sterilized PBS solution, digested with trypsin for 2-3 minutes, sucked out the digestive solution, added RPMI-1640 culture solution, Pipette to evenly disperse the cells and adjust the cell concentration to 3×10 4 / ml, were added to 96-well culture plate, 200μL per well. The culture plate was placed in a carbon dioxide incubator, cultivated at 37°C and 5% carbon dioxide for 24 hours, and then taken out and added with the test drug.

[0046] The cell lines are grouped according to the concentration of chemotherapeutic drugs: chemotherapeutic drug DDP5-500 μg / ml, the concentration of chemotherapeutic drugs refers to its highest peak concentration in the human body, and the range of 1 / 10-10 times of t...

Embodiment 2

[0053] Synergistic effect test of Shengmai injection on the chemotherapy drug GEMZAR of human colon cancer cell Lovo:

[0054] The cell lines were cultured according to the above method and grouped according to the concentration of chemotherapeutic drugs.

[0055] Place the culture plate in the incubator, take it out at 24, 36, and 72 hours respectively, suck out all the culture solution with a vacuum pump, add 20 μL of MTT to each well, continue to cultivate for 4 hours, take it out, add 200 μL DMSO to each well, shake to dissolve the precipitate, and use enzyme labeling The optical density was measured by an instrument at a wavelength of 570nm, and the tumor cell inhibition rate was calculated according to the above formula.

[0056] The results showed that when Shengmai Injection was combined with large, medium and small doses (i.e. taking 1 / 10, 1, 10 times the amount of chemotherapeutic drug GEMZAR combined with Shengmai Injection), the tumor inhibition rate was significan...

Embodiment 3

[0061] Synergistic effect test of Shengmai injection on the chemotherapy drug HCPT of human colon cancer cell Lovo:

[0062] The cell lines were cultured according to the above method and grouped according to the concentration of chemotherapeutic drugs.

[0063] The chemotherapeutic drug HCPT2-200μg / ml, the drug concentration refers to its highest peak concentration in the human body, and the range of 1 / 10-10 times the highest peak concentration is selected, and 7 groups (groups 1 to 7) are set up, and the ratio is 1:2. :5:10:20:50:100. Shengmai injection was diluted 10 times, the 8th, 9th, and 10th groups were respectively 1 / 10, 1, and 10 times of the highest peak concentration of chemotherapy drugs in the body and the superimposition of the dilution of Shengmai injection, and the 11th group was added dropwise with simple Shengmai injection. Diluents for injections. Each drug concentration was inoculated into 3 duplicate wells, and 20 μL of drug solution was added to each w...

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Abstract

The present invention relates to Chinese medicine, and is the use of pulse-activating injection in preparing synergistic medicine for treating tumor. Based on the features of clinical chemotherapy, the present invention combines the use of pulse-activating injection and chemotherapeutic medicine in treating tumor and shows the obvious synergistic effect of pulse-activating injection to chemotherapeutic medicine, especially 5-Fu, MTX, DDP, etc. in cancer cell. The present invention expands the indications of pulse-activating injection, and uses it as the synergistic medicine in the clinical treatment of colon cancer, liver cancer, breast cancer and cancer of pancreas.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine, and relates to the application of traditional Chinese medicine Shengmai injection in the preparation of synergistic drugs for treating tumors. In particular, it relates to the use of traditional Chinese medicine Shengmai injection in the preparation of synergistic drugs for treating malignant tumors. Background technique [0002] The traditional Chinese medicine Shengmai Injection is derived from the ancient prescription "Shengmaisan", which is a national essential medicine and a national protected variety of traditional Chinese medicine, and is an essential Chinese patent medicine for emergencies in traditional Chinese medicine. The production standard number of the enterprise is WS 3 -B-2865-98. The protected variety number of traditional Chinese medicine is ZYB2071998054-3. [0003] The active ingredients of Shengmai Injection are composed of red ginseng, Ophiopogon japonicus, an...

Claims

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Application Information

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IPC IPC(8): A61P35/00
Inventor 陈震孙晓波刘鲁明陈忠梁廖进明张正光康爱仙乐国祥胡春湘
Owner HEHUANG PHARMA SHANGHAI
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