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Method for cultivating salt-resistant tomato plant

A breeding method and salt tolerance technology, applied in the field of crop breeding, can solve the problems of narrow genetic background, salt tolerance can not meet the requirements of development and utilization, difficult to find adversity tolerance genes, etc., to achieve low safety hazards, low cost, Easy-to-master effect

Inactive Publication Date: 2004-04-28
SHANGHAI JIAO TONG UNIV
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AI Technical Summary

Problems solved by technology

[0002] Tomato is a strictly self-pollinated plant. Long-term artificial domestication and sexual hybridization have narrowed its genetic background. Some stress-tolerant genes are hard to find in its cultivated species.
[0003] Although there are certain differences in salt tolerance among crop varieties, their salt tolerance is still far from meeting the requirements for the development and utilization of large areas of saline-alkali land and seawater, which accounts for 97.3% of global water volume.

Method used

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Examples

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Embodiment 1

[0019] Taking the parent material 'Xianfeng' (Lycopersicon esculentum 'xianfeng') of Shanghai's main cultivars as the object of mutagenesis, soak the seeds in clean water for 24 hours at room temperature, then immerse them in 70% alcohol for 20 seconds, and then wash them with 10% to 13% bleaching powder. Disinfect the surface of the saturated solution for 20 minutes, and then rinse with sterile water 4 to 5 times. Under sterile conditions, after germinating for 48 hours, inoculate on 1 / 2 MS medium to obtain sterile seedlings.

[0020] When the seedlings were 12 days old, the hypocotyls were cut into 6-8 mm long sections (with the cotyledon, shoot tip and radicle removed) as explants, and inoculated in MS+BA (benzyl adenine) 2.0 mg / L+ IAA (indole acetic acid) 0.2mg / L+30g / L sucrose+6.5-7.0g / L agar medium, at 25±1℃, light intensity 25μmol.m -2 .s -1 , cultured under a 14-hour light / 10-hour dark photoperiod. The recovery rate can reach 100% after about 15 days. Subculture onc...

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Abstract

The process of cultivating salt tolerant tomato plant includes the steps of: taking hypocotyl of aseptic seedling as explant to inoculate and culture; inoculating the callus tissues culture after twice subculture to culture medium with one third of artificial compounded sea water; inoculating green callus tissues to culture medium without sea water for 7 or 8 subcultures before transferred to culture medium with one third of artificial compounded sea water to establish salt tolerant cell line; transferring the proved salt tolerant callus tissues to differentiating culture medium without sea water to obtain reproduction seedling; pot cultivating the seedling while irrigating with one third of artificial compounded sea water for salt tolerant screening to obtain salt tolerant plant. Compared with modern genetic engineering breeding method, the present invention has low cost, simple technology, less hidden safety danger and other obvious advantages.

Description

Technical field: [0001] The invention relates to a method for cultivating salt-tolerant tomato plants, which is used for cultivating new solanaceous varieties such as tomato, eggplant and pepper, and belongs to the technical field of crop breeding. technical background: [0002] Tomato is a strictly self-pollinating plant. Long-term artificial domestication and sexual hybridization have narrowed its genetic background, and some stress-tolerant genes have been difficult to find in its cultivated species. [0003] Although there are some differences in salt tolerance among crop varieties, their salt tolerance is still far from meeting the requirements for the development and utilization of large areas of saline-alkali land and seawater, which accounts for 97.3% of the global water volume. Plant breeders, physiologists and soil scientists are collaborating on different approaches to improve salt tolerance in crop varieties. [0004] With the confirmation of the totipotency of ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 陈火英张建华
Owner SHANGHAI JIAO TONG UNIV
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