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Method of extracting effective components in kudzu vine root

A technology of effective ingredients and pueraria, applied in the directions of medical preparations containing active ingredients, separation methods, organic chemistry methods, etc., can solve the problems of waste of Chinese medicinal materials, reduce the utilization value of pueraria, affect the economic benefits of extracting pueraria, and improve the Economic benefits and the effect of improving utilization value

Inactive Publication Date: 2004-06-23
TEA RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods only extract one active ingredient of puerarin, but do not extract and separate the two active ingredients of daidzein and daidzein at the same time, so that the daidzein and daidzein in the pueraria root are not utilized, which affects the extraction of the pueraria economic benefits, reducing the utilization value of kudzu root itself, resulting in the waste of resources of Chinese herbal medicines

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] The adsorption resin is S-8, and its pretreatment is as follows: the resin is soaked in distilled water, 2% sodium hydroxide, and 2% hydrochloric acid for 40, 3, and 5 hours in sequence, washed with water until neutral, and then washed with absolute ethanol until the effluent is no longer saturated with water. Until white turbidity appears, finally wash with distilled water until the effluent is free of alcohol.

[0012] Take fresh kudzu root, dry and crush at low temperature, and pass through a 20-mesh sieve. Weigh 1Kg of crushed kudzu root material, put it into an extraction tank, add 10L of 80% ethanol, and extract at 50°C for 10 hours. Filter with a filter screen, add 6L of 80% ethanol to the filter residue to extract again, combine the two extracts, and concentrate to 2L with a low-temperature vacuum concentrator.

[0013] Take by weighing 100g S-8 resin, pack in the chromatographic column, pass the 2L concentrated solution obtained by concentrating through the re...

Embodiment 2

[0015] Adsorption resin is S-8, and its pretreatment process is identical with embodiment 1.

[0016] Take fresh Pueraria vine, dry and crush at low temperature, and pass through a 20-mesh sieve. Weigh 1Kg of pulverized Pueraria vine material, put it into an extraction tank, add 12L of 40% methanol, and extract at 50°C for 10 hours. Filter with a filter screen, add 5L of 40% methanol to the filter residue for leaching again, combine the two extracts, and concentrate to 2L with a low-temperature vacuum concentrator.

[0017] Weigh 100g of S-8 resin, put it into 2L concentrated solution and soak for 24 hours, separate the adsorption resin and the solution, put the adsorption resin into the chromatographic column, then elute with 25% methanol, and collect the eluted puerarin component. Remove the liquid, elute with 65% methanol, collect the eluate that can obtain the daidzein component, and elute with 100% methanol, collect the eluate that can obtain the daidzein component. Con...

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Abstract

The present invention is the method of extracting effective components in kudzu vine root. The present invention features that during the separation of effective components in kudzu vine root, S-8 resin is used to adsorb the effective components of the concentrated extractive and mounted in chromatographic column for elution with ethanol or methanol solution of 30-50 %, 50-80 % and 80-100 % concentration separately, and the three kinds of eluted liquid are collected separately and concentrated, crystallized and dried separately. The said process can extract and separate the three kinds of effective components, including puerarin, daidzin and daidzein simultaneously and this can raise the utilization value and make full use of the Chinese medicine resource.

Description

technical field [0001] The invention relates to a method for extracting active ingredients from kudzu root. Background technique [0002] Puerarin is a traditional Chinese medicine that contains many active ingredients, among which puerarin, daidzein, and daidzein are the main ones. Puerarin has miraculous effects in treating cardiovascular diseases and angina pectoris. It has a special role in preventing women's diseases such as breast cancer, osteoporosis, hot flashes and other symptoms. In current medical research, daidzin, daidzein and puerarin have all been paid attention to. Among the existing methods for extracting the effective components of kudzu root, there are water extraction and alcohol precipitation purification method, polyamide column chromatography purification method, water extraction method, kudzu powder processing wastewater resin separation method and the like. These methods only extract one active ingredient of puerarin, but do not extract and separate...

Claims

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Application Information

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IPC IPC(8): A61P9/10A61P19/10A61P35/00B01D15/00C07B63/00C07D311/30C07H17/07
Inventor 江和源蒋迎江用文
Owner TEA RES INST CHINESE ACAD OF AGRI SCI
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