Insecticidal crystallin BT-Cry1Ab/1Ac rapid detection test bar

Abstract

The invention is a pesticidal crystal protein BT-Cry1Ab / 1Ac fast-detecting testing strip. It uses an one-stage translational immune affinity chromatography and a monoclonal antibody and colloidal gold labeling technique, to fast detect protein BT-Cry1Ab / 1Ac in the sample. It provides a direct evidence for justifying if the sample to be detected contains transgenation plant sample (trans-Bt-Cry protein gene). The detecting result can provide a basis for quality evaluation of plant seeds, applied in the departments such as frontier defence, custom plant inspection, food safety detection to make spot and fast check on doubtful sample. The testing strip includes: high protein affinity fibrin film acts on the reacting carrier of the system and display the reacting result, the sample pad absorbs the sample to be detected and provides the proper reflecting condition for the reacting system, the colloidal gold pad is a solid-phase carrier of colloidal gold labeled antibody, the water absorbing pad absorbs the sample after detected, the plastic basal plate act as the supporting plate of the reacting system, the double- and single- surface adhesive tapes at different standards fix each reacting film and water-absorbing material on the supporting plate, the transparent adhesive tape fixes the sample protective pad and the colloidal gold pad, and the color marked adhesive tape acts as the characteristic mark of the detecting strip.

Description

Technical field: [0001] The invention relates to a detection technology and a detection strip for detecting transgenic protein, especially BT-Cry1Ab / 1Ac, and belongs to the technical field of bioengineering. Background technique: [0002] Bt (Bacillus thuringiensis) is the abbreviation of Bacillus thuringiensis. Bt insecticide is a kind of microbial preparation produced by culturing Bt insecticide. This insecticidal bacterium produces spores and forms a protein toxin during growth and development. When observed under a microscope, it is usually an irregular crystal, called a parasporal crystal. When the pest eats paraspore crystals or spores, in the alkaline environment of the pest's intestine, the paraspore crystal dissolves, and then is enzymatically hydrolyzed by protease, releasing a substance that is highly toxic to larvae such as Lepidoptera, Diptera, and Coleoptera. Toxic molecular fragments of the poisonous effect bind to specific receptors on the surface of the dig...

AI Technical Summary

Problems solved by technology

Among these three methods, the biological test method uses insects to conduct experiments, which requires uniform insect sources, insect ages, uniform sampling locations, and uniform feeding conditions. The test takes up a lot of space and takes a long time. The variation is large, and it is difficult to make horizontal and vertical comparisons, so it is rarely used now; DNA testing only detects genes, not protein levels, and has no guiding significance for the level of resistance, and the method is complicated, the steps are cumbersome, and false positives are high. It is not suitable for detection and on-site detection of a large number of samples; the immunoassay method uses a highly specific antibody-antigen reaction to achieve rapid detection of BT-Cry1Ab and BT-Cry1Ac in transgenic plant samples. This method is convenient, practical, fast and economical. It is the development direction of transgenic BT-Cry1Ab / 1Ac protein detection

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