Rapid testing method for ayfactin fungal antibiotic
A fungal antibiotic and detection method technology, applied in the field of microbiology, can solve the problems of high price and uneconomical initial screening of samples, and achieve the effect of less dosage
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Embodiment 1
[0028] The applicant uses the methylene blue method of the present invention (see the method described above in this description for specific operations) and the agar block method (see the method shown in the references of this description about this method) (contrast) to 600 samples. Preliminary screening found that there were 69 samples with antifungal activity screened by the methylene blue method of the present invention, and there were 54 samples with antifungal activity screened by the classic agar block method (contrast), and these 54 samples are completely contained in the previous 69 samples, see Table 2. As can be seen from Table 2, the method of the present invention is more sensitive than the agar-digging block method, and when the sample cannot detect activity with the screening of the present invention, it cannot be detected with the agar-digging method; when the sample is screened with the agar-digging method, it can be detected When it is active, it must be scr...
Embodiment 2
[0037] Example 2: Screening of Marine Microbe Anti-C.albicans Activity
[0038] The applicant collected samples of sea mud, mangrove soil, and seaweed from the coast of Hainan Island, China, and separated marine bacteria (including actinomycetes) and marine fungi (mainly filamentous fungi) from them, and obtained cell-free samples by fermentation, centrifugation or filtration. Fermentation supernatant, with C.albicans as the target bacteria, is screened by the present invention. Table 3 shows the measurement results of 94 marine microbial fermentation supernatant samples in one of the 96-well plates. OD 630 Samples > 1 were considered to have anti-C.albicans activity. As can be seen from Table 3, in 94 marine microbial samples to be tested (remove a growth control well and blank control well), there are 22 samples of OD 630 >1 (underlined part in Table 3), it can be used as the starting bacteria for further screening.
[0039] Table 394 Anti-assay resul...
Embodiment 3
[0050] Example 3: Quantitative analysis of marine microorganism anti-C.albicans activity
[0051] Do standard curve by quantitative analysis method described in the present invention, as figure 1 shown. Samples were determined under the same conditions, as shown in Table 3. A in Table 3 2 Hole OD 630 = 1.523, from figure 1 It can be drawn that its activity is equivalent to the standard antibiotic (fluconazole) whose concentration is 8 μg / mL; and as in Table 3, the OD of E2 hole 630 = 1.39, similarly can be obtained from figure 1 It was found that its activity was equivalent to that of a standard antibiotic (fluconazole) at a concentration of 4 μg / mL. From these two examples, as long as the standard antibiotic curve is used, this method can quickly pass the OD of the sample. 630 Perform quantitative analysis.
[0052] Note: The sample source and preparation method of this embodiment are the same as in Example 3.
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