Method for extracting amylose of desert algae

A technology of desert algae and polysaccharides, which is applied in the field of extracting polysaccharides from desert algae, can solve the problems of high cost of cultivating spirulina and macroalgae, and achieve the effect of fast economy, convenient operation and easy method

Inactive Publication Date: 2005-01-12
INST OF AQUATIC LIFE ACAD SINICA
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Problems solved by technology

[0004] Most domestic algae polysaccharides are extracted from spirulina and macroalgae, and their polysaccharide content is mostly lo

Method used

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Embodiment Construction

[0025] The specific implementation steps of extracting desert algae polysaccharide are as follows:

[0026] A. Preparation of culture medium:

[0027] Add 1.5g of sodium nitrate, 0.04g of dipotassium hydrogen phosphate, 0.075g of magnesium sulfate, 0.036g of calcium chloride, 0.006g of citric acid, 0.006g of ferric ammonium citrate, and disodium salt of ethylenediaminetetraacetic acid (EDTA) per liter of water 0.001g, sodium carbonate 0.02g, trace element solution A51ml;

[0028] B. Cultivation of desert algae:

[0029] The cultivation of desert algae is a very important condition for determining the yield of polysaccharides. Therefore, the whole growth process of desert algae should be cultivated according to the following conditions.

[0030] (1) First-level culture: the original seed of the test tube is inserted into the triangular flask (150-200ml), cultured statically or aerated, and the airflow is adjusted to 2.5L.min during aerated culture -1 , the airflow passes th...

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Abstract

The invention discloses a method for extracting desert algae polysaccharide. Said method includes the following steps: firstly, adopting sodium nitrate, dipotassium hydrogen phosphate, magnesium sulfate, calcium chloride, citric acid, ammonium ferric citrate, disodium EDTA, sodium carbonate and minor element solution A5 to prepare culture liquor of desert algae according to a certain ratio; then controlling temperature, light-intensity and aeration condition of the culture liquor to make culture of desert algae; the third step is preparation of desert algae powder; The fourth step is extraction of desert algae polysaccharide; and the fifth step is purification, harvest and drying of desert algae polysaccharide; and the fifth step is purification, harvest and drying of desert algae polysaccharide. Said method is easy to implement, convenient in operation.

Description

technical field [0001] The invention relates to the field of biotechnology, and more specifically relates to a method for extracting polysaccharides from desert algae. Background technique [0002] At present, the utilization of desert algae generally only stays in artificial algae crusts, and the development of other applications of desert algae is almost blank, and even less is involved in the application and development of desert algae polysaccharides. The reason why desert algae are used to form artificial algae crusts for desert control is that it is rich in algal polysaccharides, and the polysaccharide content is generally more than 25% of its dry weight, and some even reach more than 45%. [0003] Algae polysaccharides can change the flow characteristics of the solution, increase the viscosity of the solution, form a stable gel, and can be used as a flocculant; the hydrophilicity and hydrophobicity of the polysaccharide macromolecules can be ...

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Application Information

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IPC IPC(8): C08B37/04
Inventor 刘永定谢作明沈银武黄泽波胡春香李敦海陈兰洲
Owner INST OF AQUATIC LIFE ACAD SINICA
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