Antigen epitope of gold staphylococcus virulence factor regulatory protein and its mimic epitope and use thereof

A technology of Staphylococcus aureus virulence factor and regulatory protein, applied in the field of two antigenic epitopes and their mimetic epitopes, can solve the problems of inability to determine antigenic epitopes, difficult to carry out research, difficult and other problems

Inactive Publication Date: 2005-01-26
INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For some antigens that are difficult to obtain or have not yet been identified, it is diffic...

Method used

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  • Antigen epitope of gold staphylococcus virulence factor regulatory protein and its mimic epitope and use thereof
  • Antigen epitope of gold staphylococcus virulence factor regulatory protein and its mimic epitope and use thereof
  • Antigen epitope of gold staphylococcus virulence factor regulatory protein and its mimic epitope and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1. Preparation and purification of TRAP protein polyclonal antibody

[0027] The purified TRAP protein was immunized to rabbits to prepare polyclonal antibodies. An affinity column coupled with TRAP protein was used to separate anti-TRAP IgG from polyantibody serum. SDS-PAGE electrophoresis results showed that the purity of the purified IgG was >90%, and the ELISA results showed that the titer was 1×10 5 Above (see attached figure 1 In the figure, 1 represents the low molecular weight protein standard, and 2 represents the purified TRAP polyclonal antibody).

Embodiment 2

[0028]Example 2. Screening of mimotopes of TRAP polyclonal antibody

[0029] First, 100μl of purified TRAP polyclonal IgG was coated on the enzyme-linked plate and placed at 4°C overnight. After blocking with 2% gelatin for 1 hour, add the phage dodecapeptide library, incubate at room temperature for 1 hour, wash the non-specifically bound phage with TBST (50mmol / LTris-HCl, 0.1% TWEEN20, pH7.5), and then use 0.2mmol / L Glycine-HCl pH2.2 eluted the specifically bound phage, and the eluate was neutralized with 1mmol / L Tris-HCl pH9.0. According to the method provided by the kit, determine the titer of the phage in the eluate, and use the titer of the eluted phage of the uncoated target protein as a control to determine the input-output ratio. At the same time, the eluted phage bound to TRAP antibody IgG was amplified, and its titer was measured for the next round of screening. After 3 rounds of screening, the measured input and output have been significantly improved. The enriched ph...

Embodiment 3

[0030] Example 3. Comparison of the screened antigen mimic epitope sequence 1 and the expressed TRAP sequence

[0031] Analyzing the screened sequence and the original sequence of the expressed TRAP protein, it is found that the sequence of the antigen mimic epitope sequence 1 is similar to the amino acid sequence 21-34 of the TRAP protein:

[0032] Antigen mimotope sequence 1 NP LH HE HA TG W T

[0033] TRAP protein primary sequence 21 NP T HO LFQF SA SD T 34

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PUM

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Abstract

The invention relates to an antigen epitope of gold staphylococcus virulence factor regulatory protein and its mimic epitope, wherein the amino acid sequence of the antigen epitope is NPTHQLFQFSASDT or SYFERYLYPIKE, the amino acid sequence of the analogue epitope is XPXHHQHXTGFT or SWFDXXLYPXXX, X is any one of the 21 known natural L-type amino acid residue or its D-type isomers. The antigen epitope or its analogue epitope can be applied in preparing staphylococcus aureus resistant medicament.

Description

Technical field [0001] The present invention relates to antigen epitopes and mimic epitopes, in particular to two antigen epitopes and mimotopes of Staphylococcus aureus (Staphylococcus aureus) virulence factor regulatory protein. The present invention also relates to the application of polypeptides consistent with these epitope amino acid sequences in the preparation of drugs or vaccines against Staphylococcus aureus infection. Background technique [0002] Staphylococcus aureus is a kind of common gram-positive pathogenic bacteria. It is one of the main microorganisms that cause fatal diseases such as burns and war infections, pneumonia, endocarditis, sepsis, and toxic shock. The number of people infected with S. aureus in the hospital alone exceeds millions every year. At present, the clinical treatment of Staphylococcus aureus mostly adopts the combined use of antibiotics, but the effect is not ideal. Since Staphylococcus aureus is very easy to develop drug resistance and the...

Claims

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Application Information

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IPC IPC(8): A61K38/16A61K39/085A61P31/04C07K7/08C07K14/31C07K14/315
CPCC07K14/315A61P31/04
Inventor 邵宁生杨光柳川高亚萍董洁丁红梅沈倍奋
Owner INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
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