Non-pathognic ralstonia solanacearum strain of transferred bacteriophage trp574 gene, and preparation method for and application of non-pathognic ralstonia solanacearum strain

Abstract

The invention discloses a non-pathognic ralstonia solanacearum strain of a transferred bacteriophage trp574 gene, and a preparation method for and application of the non-pathognic ralstonia solanacearum strain. The strain is collected in the Guangdong Microbial Culture Collection Center on July 15, 2020, and the collection number is GDMCC No: 61049. According to the non-pathognic ralstonia solanacearum strain, through a molecular biology technique, a bacteriophage trp574 gene is introduced into the ralstonia solanacearum to obtain transgenic ralstonia solanacearum engineering bacteria; and theengineering bacteria lose pathogenicity, but have relatively high colonization capacity in rhizosphere soil and tobacco plant bodies, and show the prevention and treatment effect on the tobacco bacterial wilt.

Description

technical field [0001] The invention relates to the technical field of Ralstonia solanacearum control technology, more specifically, relates to an apathogenic Ralstonia solanacearum strain transfected with phage trp574 gene and its preparation method and application. Background technique [0002] Tobacco bacterial wilt caused by Ralstonia solanacearum (commonly known as Ralstonia solanacearum) is a devastating disease in tobacco production. It has exploded in the Yangtze River Basin and the south of my country, bringing huge losses to the tobacco industry. Economic losses. R. solanacearum has a wide range of hosts, loves high temperature, and has a complex population, so it is very difficult to control. Production is generally based on agricultural control, disease-resistant breeding and chemical control, but the effect is not very satisfactory. Using disease-resistant varieties to prevent and control bacterial wilt is an economical and easy-to-operate measure. However, at ...

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Problems solved by technology

The chemical control of bacterial wilt is more difficult, because there is no ideal chemical agent at present. The agricultural streptomycin that has been used for a long time has now withdrawn from the market. The application of preparations has a certain effect on delaying and slowing down the occurrence of bacterial wilt, but it cannot fundamentally solve the problem of bacterial wilt control, and the use of a large number of chemical agents will lead to drug resistance of pathogenic bacteria and environmental pollution. Therefore, bacterial wilt The biological control of blight has received extensive attention at home and abroad

[0003] Among them, utilizing non-pathogenic R. solanacearum strains to prevent and treat crop bacterial wilt is one of the key points of research in the biological control of R. solanacearum. In the prior art (Dong Chun, Zeng Xianming, Liu Qiongguang. Utilizing non-pathogenic R. Research on bacterial wilt control of tomato bacterial wilt [J]. Journal of South China Agricultural University, 1999, 020(004): 1-4.; Chen Qinghe, Weng Qiyong, Hu Fangping. Control effect of non-pathogenic bacterial wilt on tomato bacterial wilt [J]. Chinese Journal of Biological Control, 2004,20(1):42-44.) Although there are reports on the successful use of non-pathogenic R. solanacearum for the control of R. solanacearum, the control effect still needs to be improved. In the prior art, methods such as ultraviolet mutagenesis, plant tissue isolation, and knockout of pathogenic genes are mainly used to obtain non-pathogenic strains of R. solanacearum. Reports about R. solanacearum

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