Complete virus gene engineering vaccine of aftosa and its preparation method

Abstract

The invention discloses a kind of foot-and-mouth disease genetic engineering vaccine and its the preparing method.Its structure expression is: Entire virus genetic engineering vaccine: Phage T4-Soc-Hoc-FMDV-P1; Asian unit genetic engineering vaccine: Phage T4-Soc-Hoc-FMDV-F3.The preparing method is: Takes O blood serum foot-and-mouth disease virus poisonous, extracts total gene group RNA; From blood serum poisonous DNA measure foreword to synthesizes two DNA nucleic acid probe;takes the probe as the directing thing, carries on Rt-pcr chain type DNA expand increase response, obtains the viral granule P1gene;gene recombinate the P1gene and the T4bacteriophage material particle, pRH-Soc, obtains T4bacteriophage conformity material particle pRH-Soc-P1;homology reorganizes pRH-Soc-P1with the T4bacteriophage carrier phage T4- í¸ Soc & í¸ Hoc, then enters in the backwoods coli E.coli.CR63host mycelium, obtains this invention genetic engineering vaccine phage T4-Soc-FMDV-P1.compares this invention with the deactivation entire viral vaccine, it does not have to fight fire, maintains the entire viral antigenicity but not have the infection.may inject, also may take orally, convenient, quick, the immunity effect is good.

Description

technical field [0001] The invention relates to the field of medical and health technology, in particular to a foot-and-mouth disease genetic engineering vaccine created by using a T4 phage exogenous protein high-efficiency expression and display system and a preparation method thereof. Background technique [0002] Foot-and-mouth disease is a major infectious disease that endangers cloven-hoofed animals and humans. The International Veterinary Bureau and the Ministry of Agriculture of my country have listed it as a severe infectious disease of category A. my country's "Animal Epidemic Prevention Law" stipulates that it is the first category of serious infectious diseases. Vaccine immunization is the key to preventing and treating foot-and-mouth disease. fundamental technical measures. At present, the production of attenuated vaccines for the prevention of foot-and-mouth disease has generally stopped (due to the fear that the attenuated strains will mutate and return to the s...

AI Technical Summary

Problems solved by technology

At present, scientists all over the world agree that the ideal vaccine that can prevent the attack of virulent strains of foot-and-mouth disease should consist of all fragments of VP1, VP2, and VP3 of the surface protein of the whole virus particle to participate in the formation of an immune three-dimensional structure. Genetic engineering vaccines also need to take into account the spatial structure of the whole virus Otherwise, even if the subunit genetic engineering vaccine can produce immune detection reactivity, it is difficult to produce the protection of strong virulent attack. As a result, the actual pattern of molecular biology research of worldwide foot-and-mouth disease has been formed: only research value, no practical application value