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Cony fur containing biological active substances and use thereof

A bioactive substance, rabbit skin technology, applied in application, food science, slaughtering and other directions, to achieve the effect of promoting macrophage activation, improving immune function, and inhibiting inflammation

Inactive Publication Date: 2005-05-11
VANWORLD PHARMA (RUGAO) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no method for preparing rabbit skin containing novel active substances with strong activity and high yield

Method used

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  • Cony fur containing biological active substances and use thereof
  • Cony fur containing biological active substances and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Preparation of Rabbit Skin

[0036]Use PBS (-) solution (80 grams of sodium chloride, 2 grams of potassium chloride, 11.5 grams of potassium dihydrogen phosphate, 2 grams of potassium dihydrogen phosphate dihydrate, and add water for injection to 10 with the dry acne vaccine of vaccinia virus Lister strain. l) to dissolve and shake well. Extract 0.4 ml with a needle tube and inject it into the central inner layer of the testis of the known Japanese big-eared white rabbit. On the 4th day, the cervical vertebrae were broken with force, and the scrotum was cut open under a sterile environment to remove the testicular connective tissue. Put the cut testicles into a special container with ice cubes, and then store them in a -80°C ultra-low temperature freezer. Take the testicular tissue out of the refrigerator to soften for 1 hour, grind it at 4°C, and mix it with EAGLE's medium (9.4 grams of Eagle's powder, 12.5-22.0 ml of 10% sodium bicarbonate, 10 ml of glutami...

Embodiment 2

[0038] Example 2 Preparation of Rabbit Skin

[0039] Using the Ikeda strain of vaccinia virus and New Zealand white rabbits, the antigen subculture was prepared according to the method in Example 1.

[0040] Take out the antigenic subculture virus solution from the ultra-low temperature refrigerator at -80°C, and put it into an incubator at 30°C to slowly dissolve it. Draw 5 milliliters with a 10 milliliter needle tube, inject it into 500 milliliters of PBS(-) solution, shake well, get 10 per milliliter 8 Injection solution of virus. The hair on the back of a healthy mature New Zealand white rabbit (2.75 kg) was cut off, and the cut hair was wiped with 75% alcohol cotton ball. Inject the rabbit intradermally with the above-prepared injection solution, inject 130 places, inject 0.25 ml each time, pay attention to no water leakage, no empty injection, and no injection through the skin. The injected rabbits were bred for 4 days. The acne was good, the color changed from rosy ...

Embodiment 3

[0041] Example 3 Preparation of Rabbit Skin

[0042] Using the vaccinia virus Dairen strain and Chinese rabbits, the antigen subculture was prepared according to the method in Example 1.

[0043] Take out the antigenic subculture virus solution from the ultra-low temperature refrigerator at -80°C, and put it into an incubator at 30°C to slowly dissolve it. Draw 5 milliliters with a 10 milliliter needle tube, inject it into 500 milliliters of PBS(-) solution, shake well, get 10 per milliliter 7 Injection solution of virus. The hair on the back of a healthy mature Chinese benign rabbit (2.0 kg) was cut off, and the cut hair was wiped with 75% alcohol cotton ball. Inject the rabbit intradermally with the injection solution prepared above, inject 200 places altogether, inject 0.25 ml each time, pay attention to no water leakage, no empty injection, and no injection through the skin. The injected rabbits were fed for 3-4 days. The acne was good, the color changed from rosy to p...

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PUM

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Abstract

A rabbit hide containing novel bioactive material for preparing medicine and health-care product is produced through inoculating the cowpox virus in the body of rabbit, raising it until the cowpox is better developed in skin tissue, and killing the rabbit.

Description

technical field [0001] The invention designs a rabbit skin containing novel bioactive substances and its application. Background technique [0002] It has been reported that the extract obtained from the skin of rabbits infected with poxvirus has a therapeutic effect on allergic diseases, has an analgesic effect on pain such as neuropathic pain, and enhances immunity. Yet, also do not have a kind of method for preparing the rabbit skin containing the novel active substance of strong activity and high yield at present. Contents of the invention [0003] The purpose of the present invention is to provide a kind of rabbit skin containing a novel active substance with strong yield and high yield, which can be used for the preparation of medicines and health care products. [0004] The inventor of the present invention has finally reached the above object through years of painstaking research. [0005] The rabbit skin of the present invention is prepared by the following meth...

Claims

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Application Information

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IPC IPC(8): A01K67/02A22B3/00
Inventor 张永深
Owner VANWORLD PHARMA (RUGAO) CO LTD
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