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Method for producing glabridin product

A production method and technology of glabridin, which can be applied in the fields of anti-toxic agent, organic chemistry, drug combination, etc., can solve the problems of reported and unseen glabridin production technology, etc.

Inactive Publication Date: 2005-05-18
匡军
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is no report on the production technology of glabridin in domestic and foreign public documents

Method used

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  • Method for producing glabridin product

Examples

Experimental program
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Effect test

Embodiment 1

[0016] First dry and pulverize the licorice glabra, then put the crushed licorice glabra and ethyl acetate into a reflux heating device according to the ratio of 1:10, heat and reflux together for 8 hours, filter and wash. Repeat twice, combine the filtrate and washings, concentrate, recover the solvent, and dry under reduced pressure to obtain a residue, which is a crude product.

[0017] The above crude product was ground, dissolved in a small amount of chloroform, and the solution was carefully added to a pre-treated silica gel column filled with silica gel (the silica gel was produced by Qingdao Ocean Chemical Factory). With chloroform, acetone gradient elution. The effluent was examined by silica gel thin-layer chromatography detection (color development with dilute sulfuric acid). The target liquid was collected, concentrated under reduced pressure, and dried in vacuo. Add a small amount of ethanol to dissolve the residue, filter, and then concentrate the filtrate unde...

Embodiment 2

[0019] Dry and pulverize Glycyrrhizae glabra first, then put the crushed licorice glabra and dichloromethane into a percolator at a ratio of 1:5, percolate, and recover when a colorless liquid flows out (about 10 hours). solvent, and the residue was dried in vacuo to obtain a crude product.

[0020] The above crude product was dissolved in a small amount of chloroform, and the solution was carefully added to a pre-treated silica gel column filled with silica gel (the silica gel was produced by Qingdao Ocean Chemical Factory). With chloroform, acetone gradient elution. The effluent was examined by silica gel thin-layer chromatography detection (color development with dilute sulfuric acid). The target liquid was collected, concentrated under reduced pressure, and dried in vacuo. Add a small amount of ethanol to dissolve the residue, filter, and then concentrate the filtrate under reduced pressure and dry it in vacuo to obtain the product. After testing, the content of Glabrid...

Embodiment 3

[0022] First dry and pulverize the licorice glabra, then put the crushed licorice glabra and methylene chloride into a reflux heating device according to the ratio of 1:7, heat and reflux together for 6 hours, filter and wash. Repeat twice, combine the filtrate and washings, concentrate, recover the solvent, and dry under reduced pressure to obtain the residue, which is the crude product I.

[0023] Or put the Glycyrrhiza glabra powder and ethyl acetate into the percolation device according to the ratio of 1:7, and percolate. When the colorless liquid flows out (about 10 hours), the solvent is recovered, and the residue is vacuum-dried , to obtain crude product II.

[0024] The crude product I and crude product II obtained above were ground, dissolved in a small amount of chloroform, and the solution was carefully added to a pre-treated silica gel column filled with silica gel (the silica gel was produced by Qingdao Ocean Chemical Factory). With chloroform, acetone gradient e...

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Abstract

The present invention relates to the production process of extracting active component glabridin from common licorice as a kind of medicinal plant. The production process includes the following steps: drying and crushing common licorice, organic solvent reflux extraction or diacolation extraction, concentrating the extract liquid and decompresson drying to obtain coarse product; dissolving the coarse product in chloroform, gradient elution with mixed chloroform-acetone solvent in silica gel column, silica gel thin layer chromatography until dilute sulfuric acid development, collecting target solution, decompression concentration, vacuum drying, dissolving in ethanol, filtering, decompression concentrating the filtrate and vacuum drying to obtain the product. The product is yellow brown powder and has active component content up to 44.64 %.

Description

technical field [0001] The invention relates to biological extraction technology, in particular to a production method for extracting its active ingredient-glabridin product from natural organisms. Background technique [0002] Glabridin is a natural antioxidant, which is a flavanone compound isolated from the medicinal plant Glycyrrhiza Glabra. It has strong antioxidant and estrogenic activities, and can activate the human body SOD gene, as a cosmetic application, can resist skin aging, also known as "whitening factor". Glabridin is safe and non-toxic, and harmless to the human body even if it is used for a long time. It is usually used as a cosmetic additive and pharmaceutical raw material in industry. [0003] [0004] Glabridin [0005] (Glabridin) [0006] At present, there is no report about the production technology of glabridin in domestic and foreign open documents. Contents of the invention [0007] The purpose of the present invention ...

Claims

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Application Information

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IPC IPC(8): A61P39/06C07D493/04
Inventor 匡军
Owner 匡军
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