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Method for producing paclitaxel using isolated plant tissue

A paclitaxel and plant technology, which is applied in the biological field of pharmaceutical production, can solve the problems of low yield of target compounds, serious degradation of paclitaxel, low content of paclitaxel, etc., and achieves the effects of no pollutant discharge, low production cost and single extraction component.

Inactive Publication Date: 2005-06-22
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the above production process, the plant material has not undergone reasonable post-harvest processing, not only the content of paclitaxel in the branches and leaves is low, but also in the above production process, paclitaxel and its precursors are seriously degraded, resulting in low yield and yield of the target compound. Stability and other issues

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Collect yew branches and leaves in spring, cut them to about 20cm, take 25g cuttings of branches and leaves under sterile conditions, put them in 100mL solid modified MS medium, add 50mg.L -1 Potassium dichromate, pollution suppression, 100μmol.L -1 Methyl jasmonate (MJ) induces the production of paclitaxel and its precursors. The culture environment was sterilized by ultraviolet light, cultured in the dark at 25±1°C, and samples were taken and analyzed on the 6th day after inoculation, and the paclitaxel content increased by 67%.

Embodiment 2

[0019] Collect yew branches and leaves in the current year in autumn, cut them to about 40cm, cut 25g branch and leaf cuttings under sterile conditions, put them in 200mL solid modified MS medium, add 50mg.L -1 Potassium dichromate, inhibit pollution, 50mg / L fungal inducer Aspergillus niger cell wall polysaccharide induces the production of paclitaxel and its precursors. Incubate in dark at 25±1°C, sample and analyze on the 6th day after inoculation, the paclitaxel content increased by 60%.

Embodiment 3

[0021] Sampling in spring, cut branches and leaves to about 30cm, cut 25g branch and leaf cuttings under sterile conditions, put them in 100mL solid modified MS medium, add 50mg.L -1 Kanamycin, to inhibit pollution, 2mg / L cerous nitrate induces the production of paclitaxel and its precursors. Cultured in the dark at 30±1°C, sampled and analyzed on the 3rd day after inoculation, the paclitaxel content increased by 37%.

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PUM

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Abstract

The invention provides a method for producing paclitaxel using isolated plant tissue which comprises the following steps, (1) selecting and collecting the fresh branches and leaves of the current year yew, (2) cleaning, airing and tailoring, (3) carrying out cuttage or branch and leaf chopping under the condition of darkness, coldness and external nutrition, cultivating the tissue, (4) carrying out inducement treatment to the explant during culturing process, (5) adding precursor substance for yew alcohol biological synthesizing into culture media, (6) low-temperature disintegrating and organic solvent lixiviating through conventional processes. The invention can be applied for substantially increasing the yield of yew alcohol.

Description

technical field [0001] The invention belongs to a biological method for producing medicines, in particular to a method for producing paclitaxel by utilizing branches and leaves of yew plants. Background technique [0002] At present, due to its unique tumor-suppressing mechanism and remarkable anticancer activity, paclitaxel ranks first in the market share of anticancer drugs, accounting for 22% of the market share of the world's main anticancer drugs. In 2000, BMS company reported that paclitaxel injection (Taxol TM ) sales of 15.92 billion US dollars, with an average daily sales of more than 4.3 million US dollars. Natural paclitaxel is mainly derived from Taxus plants, which are evergreen trees or shrubs. There are about 11 species in the world. my country is one of the native places of Taxus, and there are 4 species and 1 variant, namely Tibetan Taxus ( T. wallichiana), Northeastern yew (T. cuspidata), Yunnan yew (T. yunnanensis), Chinese yew (T. chinensis) and southern...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H3/04A01H4/00C07D305/14
Inventor 余龙江刘智赵春芳付春华杨英杨秦李丽琴
Owner HUAZHONG UNIV OF SCI & TECH
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