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Molecular surface imprinting polymer preparing method

A technique of imprinting polymers and surface imprinting, applied in chemical instruments and methods, biological testing, semi-permeable membrane separation, etc., can solve problems such as deposition of complementary functional groups, difficulty in large-scale preparation of imprinted materials, loss of flexibility, etc. Achieve maximum application value, improve adsorption capacity and selectivity effect

Inactive Publication Date: 2005-06-29
SHANGHAI JIAO TONG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this method, since the flexibility of the surface groups of protein molecules is basically lost after dehydration, complementary functional groups cannot be deposited near the functional groups of charge, hydrophobicity, hydrogen bond acceptors and donors on the protein surface. It is difficult to improve the performance of molecular recognition materials by introducing functional groups, and on the other hand, it is also difficult to prepare imprinted materials on a large scale
Therefore, fundamentally improving the performance of imprinted polymers is a scientific and technological challenge

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Surface imprinted polyacrylamide with hepatitis B e antigen as template molecule

[0020] In the present invention, the imprinted polymer prepared with HBe antigen as a template is represented by MIPe, and the imprinted polymer prepared without any template molecule is represented by MIPc. The steps and results are as follows:

[0021] Immobilization of a template protein molecule

[0022] Set the concentration to 10 4 The hepatitis B e antigen (HBe) of mg / ml was added in the microwell of the 96-well microtiter plate, and kept at 4°C overnight. Rinse off unbound template molecules with 0.5% TWEEN-normal saline, and dry the microtiter plate for later use.

[0023] Incubation of bifunctional monomers with immobilized proteins to form complexes

[0024] Mix 10ml of acrylamide (50%), N,N'-methylenebisacrylamide stock solution (8%), and methacrylic acid (6%) evenly, add 400ul of ammonium persulfate and 200ul of N,N,N',N '-Tetramethylethylenediamine (TEMED), m...

Embodiment 2

[0031] Example 2: Surface-imprinted poly(propylene alcohol) using hepatitis B e antigen as a template molecule

[0032] The steps and results are as follows:

[0033] Immobilization of a template protein molecule

[0034] Same as Example 1

[0035] Incubation of bifunctional monomers with immobilized proteins to form complexes

[0036] Add 300ul ammonium persulfate and 100ul N,N,N',N'-tetramethylethylenediamine (TEMED) to 10ml of allyl alcohol (20%), styrene (12%), mix well, add 0.4ml to the bag In the microwells of the ELISA plate with HBe molecules, incubate with the template molecules immobilized on the surface of the carrier for 30 minutes, and the template protein molecules combine with the functional monomer styrene to form a complex, so that the functional monomers are distributed on the surface of the template protein molecules.

[0037] Tri-initiated copolymerization of fixed functional monomers

[0038] After incubation for 30 minutes, the polymerization reaction...

Embodiment 3

[0044] Example 3: Surface imprinted polystyrene with hepatitis B c antigen as template molecule

[0045] The steps and results are as follows:

[0046] Immobilization of a template protein molecule

[0047] Same as Example 1

[0048] Incubation of bifunctional monomers with immobilized proteins to form complexes

[0049] Add 300ul ammonium persulfate and 120ul N, N, N', N'-tetramethylethylenediamine (TEMED) to 10ml styrene and methacrylic acid (5%), mix well, add 0.4ml to the coated HBe molecule Incubate with the template molecules immobilized on the surface of the carrier for 30 minutes in the microwells of the microtiter plate, and the template protein molecules combine with the functional monomers to form a complex, so that the functional monomers are distributed on the surface of the template protein molecules.

[0050] Tri-initiated copolymerization of fixed functional monomers

[0051] After incubation for 30 minutes, the polymerization reaction occurred, and the imp...

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PUM

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Abstract

A method for preparing molecule surface engram polymer has the steps as follows: fix the pattern molecule on the carrier surface, wash that not being combined by buffer solution, dry the carrier; hatch the synthesized monomer, cross linking reagent and functional monomerand the pattern molecule fixed on the carrier surface for combining pattern protein molecule and function monomer to be compound and distributing function monomer on the surface of pattern protein; add initiator to cause polymerization, fix the compound formed by pattern molecule and function monomer on the polymer surface; wash the engram polymer for clearing away the pattern molecule remarked in it, finally acquire the product. The invention makes the engram of molecule on the surface of material, which eliminates hard washing of pattern molecule embedded in polymer as well as makes the pattern molecule engram, and greatly advances the recognition to substance and dynamic performance.

Description

technical field [0001] The invention relates to a method for preparing a molecular surface imprinted polymer, in particular to a method for preparing a surface imprinted polymer capable of recognizing template molecules. It is used in the field of molecular recognition, detection and separation and purification. Background technique [0002] Molecular imprinting refers to the process of complementary imprinting the shape of specific molecules and the properties of charges, hydrophobicity, hydrogen bond acceptors and donors distributed on the surface of molecules into polymers, which can retain the properties of the printed molecules. Imprinting of shape and molecular surface properties. The imprint in the polymer has a specific corresponding relationship with the imprinted molecules, and has the effect of memory template molecular shape, so that the imprinted polymer material has the ability to recognize specific molecules. [0003] In recent years, there have been many re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D71/06C08J5/00G01N33/53G01N33/68
Inventor 李荣秀郭德宪王霆
Owner SHANGHAI JIAO TONG UNIV
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