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Extraction method of ginkgolactone B and ginkgo lactone in ginkgo leaf

A technology for ginkgolide and bilobalide, which is applied in directions such as organic chemistry, can solve the problems of high cost, high production cost, non-separation of ginkgolide and the like, and achieves the effects of simple method and low cost

Active Publication Date: 2005-08-10
DALIAN SHUANGDI TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This extraction method uses more than 10 kinds of organic reagents, wherein carbon tetrachloride, carbon dichloride, benzene, lead acetate, etc. are poisonous, and the production cost is high, and ginkgolides are not separated. According to patent reports, most of them are extracted with ethanol, purified by resin adsorption, separated by silica gel column, and some are also extracted with toxic reagent chloroform, but there is no report on the simultaneous isolation of ginkgolide B and bilobalide
Hefei University of Technology uses Ginkgo biloba extract (containing 24% ginkgo flavonoid glycosides, 6% ginkgolides), extracts ginkgolides with acetone-petroleum ether, and then extracts ginkgolides with supercritical carbon dioxide. Although the method is simple, it is expensive. High, and ginkgolide does not separate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0007] A, with the dry green ginkgo leaf of purchasing, dry at 65 or 68 or 70 or 73 or 75 ℃, remove impurity, pulverize, cross 60 mesh sieves, weigh 60kg, put into extraction tank, add 600L50% ethanol, in Under stirring, extract at 70°C for 90 minutes, stop stirring, filter, repeat the extraction twice, and concentrate under reduced pressure. When the recovered ethanol content is below 20%, stop the concentration, put it into a storage tank, add 100L of purified water, and precipitate for 20 hours. Take the supernatant for centrifugation and separation, and put the centrifuged supernatant on the ADS-15 adsorption column (when the effluent is dark blue when tested with 1% ferrous sulfate, it means that the column is saturated and the column is stopped). Wash through the column with purified water, and when the effluent is clear, elute with 75% ethanol (about 120L), collect the eluate, recover ethanol under reduced pressure, and when it is concentrated to about 40L, stop heating ...

Embodiment 2

[0012] A. Dry the purchased dry green ginkgo leaves at 65 or 68 or 70 or 72 or 75°C, remove impurities, pulverize, pass through a 55 or 58 or 60 or 62 or 65 mesh sieve, weigh 60kg, and put it into the extraction tank Add 550L of 55% ethanol, under stirring, extract at 70°C for 120 minutes, stop stirring, filter, repeat the extraction once more, filter, combine the two filtrates, concentrate under reduced pressure until the recovered ethanol content is below 20%, stop heating, Add 200L of purified water, precipitate for 22 hours, take the supernatant and centrifuge, and put the centrifuged clear solution on the ADS-15 adsorption column (when the effluent is dark blue with 1% ferrous sulfate, it indicates that the column is saturated, stop upper column). Wash the column with purified water, and when the effluent is clear, elute with 75% ethanol (about 120L), collect the eluate, concentrate under reduced pressure, recover ethanol, stop heating when it is concentrated to about 40L...

Embodiment 3

[0017] A. The purchased dry green ginkgo leaves are further dried at 65 or 68 or 70 or 72 or 75°C, removed, crushed, passed through a 55 or 58 or 60 or 63 or 65 mesh sieve, weighed 60kg, and put into an extraction tank , add 500L of 60% ethanol, under stirring, extract at 70°C for 120 minutes, stop stirring, filter, repeat the extraction once more, combine the two extracts, concentrate under reduced pressure until the recovered ethanol content is below 20%, stop heating, add 200L of purified water, precipitate for 24 hours, take the supernatant and centrifuge, and put the centrifuged clear liquid on the ADS-15 adsorption column (when the effluent is tested with 1% ferrous sulfate and shows a dark blue reaction, it means that the column is saturated, stop loading the column ). Wash the column with purified water, and when the effluent is clear, elute with 80% ethanol (about 120L), collect the eluate, recover ethanol under reduced pressure, and when it is concentrated to about 4...

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Abstract

A process for extracting ginkalide B and biobalide from gingko leaf includes such steps as baking gingko leaves, removing impurities, breaking, extracting in aqueous solution of alcohol, purifying by adsorptive column, extracting gingkalide purifying by alumina column, step crystallizing and recrystallizing.

Description

technical field [0001] The invention belongs to a plant extract and relates to a method for separating and extracting ginkgolide B and bilobalide from ginkgo leaves. The ginkgolide B and bilobalide extracted by the invention are suitable for treating diseases caused by platelet activating factors and neurological diseases. Background technique [0002] Ginkgolide and bilobalide are the only special substances found in Ginkgo biloba. Due to the complex spatial structure and variable chirality, the synthesis is very cumbersome. Currently, they can only be extracted from Ginkgo biloba leaves. Because of their specific effects in treating diseases, the research at home and abroad is relatively hot. There is no report on the simultaneous separation and extraction of ginkgolide B and bilobalide in foreign countries. There are reports on the extraction of ginkgolide, such as the Japanese Patent Publication Ping 3-24084 , Ginkgo biloba leaves are extract...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D493/00
Inventor 王敬勉廖德胜
Owner DALIAN SHUANGDI TECH
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