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Method for preparing protein polypeptide through enzymolysis of cow milk

A production method and technology of enzymatic protein hydrolysis, which is applied in the field of production of milk enzymatic protein polypeptides, can solve the problem that the thorough enzymatic hydrolysis scheme is not perfect, etc., and achieve the effects of regulating the body's immunity, easy absorption and utilization, and reduced dosage

Active Publication Date: 2005-08-31
黑龙江福康生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Obviously, the current thorough enzymatic hydrolysis scheme of skim milk is not perfect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Take 100 kg of milk from cows on the 45th day of lactation, centrifuge and degrease, slowly add 4N NaOH while stirring to keep the pH of the colostrum liquid at 8.0-8.5, and at the same time heat up to 50-55°C until the liquid is uniform particles. Then 100 grams of Aspergillus protease (activity is calculated as 10000 units / gram) solution is evenly added in the feed liquid, and the above conditions are continued, and the enzyme reacts for 2 hours, or at least 1 hour. After the end of the aspergillus proteolysis reaction, the feed liquid was rapidly cooled to 45° C., and 5 grams of trypsin (activity was calculated as 2500 units / mg) diluted was evenly mixed into the second step of the enzyme reaction, and the enzyme reaction was performed for 1 hour. Then heat to 80°C for 15 minutes. After the whole enzyme reaction is finished, the enzymatic solution is taken for ultrafiltration purification and concentration, and the pH of the ultrafiltered polypeptide solution is <7.5...

Embodiment 2

[0021] Collect 100 kg of milk secreted by cows on the third day. After centrifugal degreasing, while stirring, slowly add 4N NaOH to keep the pH of the colostrum feed liquid at 8.0-8.5, and at the same time heat up to 50-55°C until the feed liquid is uniformly yellow. Then 200 grams of Aspergillus protease (activity is calculated as 10000 units / gram) solution is evenly mixed into the feed liquid, and the above conditions are kept, and the enzyme reaction is performed for 2 hours, or at least 1 hour. After the aspergillus proteolysis reaction is finished, the feed solution is rapidly cooled to 45°C, and 10 grams of diluted trypsin (activity is calculated as 2500 units / mg) is evenly mixed into the second-step enzyme reaction, and the enzyme reaction is at least 1 hour. Then heat to 80°C for 15 minutes. After the whole enzyme reaction is finished, the feed liquid is taken for ultrafiltration purification and concentration, and the pH of the feed liquid after ultrafiltration is l...

Embodiment 3

[0024] Collect 100 kg of milk secreted by the cow on the tenth day. After defatting, add 6N HCl to acidify the colostrum to pH 4.7, decant the supernatant, and collect the white casein precipitate. Then the precipitate was washed three times with water to restore the original volume, and then 4N NaOH was added to completely dissolve the casein. Then, 100 grams of Aspergillus protease (activity is calculated as 10,000 units / g) solution is evenly mixed into the feed liquid. Enzyme reaction conditions: normal pressure, pH 8.0-8.5, temperature 50-55°C, time 2 hours, or at least 1 hour . After the aspergillus proteolysis reaction, the temperature of the feed liquid was rapidly cooled to 45°C, and 5 grams of trypsin (activity was calculated as 2500 units / mg) solution was evenly mixed into the second step of the enzyme reaction, and the enzyme reaction was at least 1 hour. Then heat to 80°C for 15 minutes. After the whole enzymatic reaction is finished, the enzymolyzed polypeptide...

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Abstract

A process for preparing protein polypeptide from cow milk by enzymolyzing in order to use it for regulating immunity and releasing fatigue includes such steps as defatting, fully disolving the protein contained in cow milk, respectively adding aspergillus proteinase and trypsin, enzymolyzing twice, ultrafiltering and drying.

Description

technical field [0001] The invention relates to a two-step enzymatic hydrolysis and ultrafiltration process of Aspergillus protease and trypsin, and skim milk is used as raw material to produce polypeptide substances. Fatigue effect. Background technique [0002] Cow's milk usually refers to the milk secreted by cows after normal childbirth. It is popular because of its complete nutrition and rich protein. However, the physical and chemical properties of cow's milk protein are different from human milk protein. After taking it, it is easy to coagulate when exposed to acid, and it is not easy to be digested and absorbed by infants. In addition, some cow's milk protein has allergic adverse effects on the human body. The existing production process solves this problem by thoroughly enzymatically hydrolyzing milk protein into polypeptides and amino acids. However, it has recently been found that certain peptide chain fragments of lactoglobulin, lactalbumin and casein in milk h...

Claims

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Application Information

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IPC IPC(8): C12P21/02
Inventor 颜贻谦曹道明曹美英王海霞
Owner 黑龙江福康生物科技股份有限公司
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