Non-injection preparation of growth hormone and purpose thereof
A technology for growth hormone and injection preparation, applied in the preparation of growth hormone and its application, protein preparation and its application field, can solve the problems of unacceptable, affecting biological activity, fragmentation of growth hormone peptide chain, etc., to achieve good chemical Effects on stability and biological activity
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[0032] Examples: The following examples are only used to illustrate the present invention, and should not be construed as any limitation to the present invention.
[0033] Raw materials: The reagents used in the present invention are all from SIGMA Company of the United States.
example 1
[0034] Example 1: Screening of Growth Hormone Stable Solutions
[0035] In the research design of the present invention, the basic buffer plus different combined formula solutions were used for research and screening, and finally eight groups of basic formulas were selected for the stability experiment of growth hormone in vitro. After each group of experiments is equipped, it is stored at room temperature (22-25°C) and protected from light. The chemical structure analysis and biological activity of growth hormone are carried out at one month, two months, three months and six months after storage. Determination. The experimental groups and formulation components are listed in the table below.
[0036] basic ingredients
STB-1
STB-2
STB-3
STB-4
STB-5
STB-6
STB-7
STB-8
rhGH
1mg / ml
1mg / ml
1mg / ml
1mg / ml
1mg / ml
1mg / ml
1mg / ml
1mg / ml
2mM chloride
+
0.4mM ...
example 2
[0056] Example 2: Human Trial-1
[0057] The present invention adopts STB-8, each milliliter contains 12 micrograms of growth hormone, and every 60 milliliters is filled into a spray bottle for volunteers to try out.
[0058] The choice of volunteers is mainly based on age and some age-related symptoms. These symptoms and manifestations are: (1) The trial participants seem to be easily and physically and mentally exhausted in the past one or two years, and their spirits are relatively tense, their attention is not concentrated, and their work efficiency is low. , which has not been the case in the past. (2) The trial subjects have poor sleep quality, or have insomnia, without past history. (3) After eating in the evening, I feel that my digestion is not good, and I feel bloated at night. (4) Feeling of discomfort in multiple joints or back, that is, the phenomenon of "lumbar and leg inflexibility". (5) The heartbeat is fast, generally 80 beats per minute. (6) Loss of libid...
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