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Stent carried with gene with function of preventing from restenosis in coronary artery stent

A technique for coronary artery and restenosis, which is applied to gene-carrying stents for preventing and treating coronary in-stent restenosis, and in the field of stents for preventing and treating coronary in-stent restenosis, and achieves the effect of inhibiting intimal hyperplasia and improving biocompatibility.

Inactive Publication Date: 2005-11-09
SHANGHAI FIRST PEOPLES HOSPITAL
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Problems solved by technology

However, studies have shown that the relative deficiency of apoptosis is an important reason for the formation of restenosis
Interleukin-1β-converting enzyme (ICE) gene is a common pathway of apoptosis signal transmission. It has been found that this gene can induce apoptosis of vascular SMC cultured in vitro, but whether it can inhibit intimal hyperplasia by inducing apoptosis and prevent Restenosis after PCI has not been reported

Method used

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  • Stent carried with gene with function of preventing from restenosis in coronary artery stent
  • Stent carried with gene with function of preventing from restenosis in coronary artery stent
  • Stent carried with gene with function of preventing from restenosis in coronary artery stent

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Embodiment 1

[0021] Obtain target gene ICE

[0022] Use restriction endonuclease method, chemical synthesis method, PCR amplification or RT-PCR method, genome library or cDNA library screening method to obtain the target gene ICE. The invention uses PCR amplification to obtain ICE from human nasopharyngeal carcinoma cDNA library.

[0023] Construction of human ICE clones

[0024] Use pcDNA4 / myc-His as the vector to recombine the pcDNA4-ICE eukaryotic expression plasmid; use EcoR I and XhoI double enzymes to cut the target gene fragment and pcDNA4 vector, and recombine the double sticky end link.

[0025] After the pcDNA4-ICE recombinant plasmid is transformed, amplified and identified, it is dissolved in 0.1M Tris (PH 8.0) or pure water at a concentration of 4-15mg / ml.

[0026] The pcDNA4-ICE recombinant plasmid has the sequence of sequence 1.

[0027] Preparation of pcDNA4-ICE gene-loaded scaffolds

[0028] Dissolve gelatin in water to make a 5% solution, add glutaraldehyde with 2% ge...

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Abstract

A gene-carrying scaffold based on the theory that the ICE gene transfection can suppress the generation of new endomembrane for preventing and treating the restricture inside the arteria coronaria scaffold is a gelatin coated stainless steel scaffold carrying the plasmid pc DNA4 mediated interleukin-1 beta conveyzyme (ICE) genes.

Description

technical field [0001] The invention belongs to the field of drugs and medical instruments, and relates to a stent for preventing restenosis in a coronary artery stent. In particular, it relates to a gene-loaded stent for preventing restenosis in a coronary artery stent. Background technique [0002] Stent implantation has replaced most balloon dilatation and has become the main method of coronary interventional therapy (PCI). However, in-stent restenosis seriously hinders the further development of this technology. Studies predict that gene therapy is expected to become an effective way to solve restenosis after stent implantation. According to reports, most of the current gene therapy strategies are to inhibit the proliferation and migration of smooth muscle cells (SMC). However, studies have shown that the relative deficiency of apoptosis is an important reason for the formation of restenosis. Interleukin-1β-converting enzyme (ICE) gene is a common pathway of apoptosi...

Claims

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Application Information

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IPC IPC(8): A61M31/00
Inventor 孙宝贵何奔赵军礼钟伟
Owner SHANGHAI FIRST PEOPLES HOSPITAL
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