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Method for preparing insect protein with blood-fat reducing function

An insect protein and blood lipid-lowering technology, which is applied in the field of bioengineering technology, can solve the problems of low content of active ingredients, insignificant health care function, insignificant effect, etc. The effect of increasing the content

Inactive Publication Date: 2005-11-23
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some related products have also been developed in China, but most of these products have simply processed the fly maggot protein, and have not separated various active proteins in the process of processing, and have specific effects on the specific proteins in the fly maggot protein. There is no in-depth research on what kind of health function, so there is no essential difference between the obtained nutritional dry powder and fly maggots, so that the health function of this type of product is not prominent, and the effect is not obvious
As a result, the content of active ingredients in the product is low and the pertinence is not strong

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] When the fly maggots grow to 3 days old, take 2kg of washed fresh maggots and treat them with 30W 19-22kHz ultrasonic waves for 9 minutes; continue to cultivate the treated maggots for 48 hours, during which only appropriate normal saline is supplied and food is stopped . Then after the fly maggots stopped cultivating were ground and homogenized with a colloid mill, the maggot slurry was diluted with 0.02mol / L phosphate buffer solution of pH 7.0 with 5 times of volume, centrifuged at 5000rpm for 30min, and the supernatant was taken. The supernatant was diluted with 0.02 mol / L phosphate buffer solution of pH 7.0 to a protein content of 3.5%, and ultrafiltered with an ultrafiltration membrane with a molecular weight of 100,000 Daltons to collect the filtrate. The collected filtrate was diluted with 0.02 mol / L phosphate buffer solution of pH 7.0 to a protein content of 2.5%, and ultrafiltration was performed using an ultrafiltration membrane with a molecular weight of 30,0...

Embodiment 2

[0016] When the fly maggots grow to 3 days old, take 3kg of washed fresh maggots and treat them with 50W 19-22kHz ultrasonic waves for 7 minutes; continue to cultivate the treated maggots for 72 hours, during which only appropriate normal saline is supplied and food is stopped . Then after the fly maggots stopped cultivating were ground and homogenized with a colloid mill, the maggot slurry was diluted with 0.02mol / L phosphate buffer solution of pH7.2 with 7 times of volume, centrifuged at 5000rpm for 30min, and the supernatant was taken. The supernatant was diluted with 0.02 mol / L phosphate buffer solution of pH 7.2 to a protein content of 3%, and was ultrafiltered with an ultrafiltration membrane with a molecular weight of 100,000 Daltons to collect the filtrate. The collected filtrate was diluted with 0.02 mol / L phosphate buffer solution of pH 7.2 to a protein content of 2%, and ultrafiltration was performed using an ultrafiltration membrane with a molecular weight of 30,00...

Embodiment 3

[0018] When the fly maggots grow to 3 days old, take 2kg of washed fresh maggots and treat them with 60W 19-22kHz ultrasonic waves for 6 minutes; continue to cultivate the treated maggots for 54 hours, during which only appropriate normal saline is supplied and food is stopped . Then the fly maggots or early pupae after stopping the cultivation were ground and homogenized with a colloid mill, and the maggot slurry was diluted with 0.02mol / L phosphate buffer saline solution of pH6.8 with 10 times the volume, centrifuged at 5000rpm for 30min, and the supernatant was taken. The supernatant was diluted with 0.02 mol / L phosphate buffer solution of pH 6.8 to a protein content of 2.0%, ultrafiltered with an ultrafiltration membrane with a molecular weight of 100,000 Daltons, and the filtrate was collected. The collected filtrate was diluted with 0.02 mol / L phosphate buffer solution of pH 6.8 to a protein content of 1.5%, and ultrafiltered with an ultrafiltration membrane with a molec...

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Abstract

The invention provides a method for preparing insect protein with blood-fat reducing function, which comprises cultivating house fly larva, homogenating and separating. By employing fore treatment and hyperfiltration technology suitable for mass production, the active protein content in the maggot can be increased substantially. The maggot protein prepared thereby has very strong actions of lowering serum cholesterol and triacyl glycerol.

Description

technical field [0001] The invention relates to a preparation method in the technical field of bioengineering, in particular to a preparation method of insect protein with blood lipid-lowering function. Background technique [0002] Fly maggot is an important protein resource, with a protein content of 56%, more than 16 kinds of amino acids, 14% to 16% of fat, and 20% of chitosan, and is rich in biologically active substances and nutrients. Very good edible and medicinal value. In the mid-1970s, Japan reported that a variety of antibacterial substances were extracted and isolated from fly maggots, which aroused the interest of scientists from various countries in the study of biologically active components in fly maggots. Due to the high nutritional value of fly maggots and special active ingredients, in the past 20 years, a large number of researches have been done on the development and utilization of fly maggots at home and abroad, and the use of fly maggots has shifted ...

Claims

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Application Information

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IPC IPC(8): C07K14/435
Inventor 胡洪波张雪洪彭华松
Owner SHANGHAI JIAO TONG UNIV
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