Method for preparing solution for controlling cucumber and tomato disease using albizzia julibrissin extract
An extract and the technology of using Albizia julienne, applied in the field of pesticides, can solve the problems of no research reports on the prevention and treatment of plant diseases by leaves of Albizia julienne, no research on leaf extracts, etc., and achieve the effects of easy artificial cultivation, good preventive effect, and simple extraction process
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Embodiment 1
[0013] The weight percent of water agent for controlling cucumber and tomato diseases: 0.95% of Albizia Julibrissin leaf extract, 99.05% of distilled water.
[0014] Weigh 20 g of Albizia Julibrissin leaf powder (20 mesh particles), immerse and extract 3 times with 200 ml of 95% ethanol at room temperature, each time for 24 hours, concentrate under reduced pressure and recover the solvent to obtain 3.1 g of dry matter; use ultrasonic waves to suspend the dry matter with 50 ml of water, Equal volumes of petroleum ether, ethyl acetate, and water-saturated n-butanol were extracted 5 times in sequence, and the n-butanol phase extract was concentrated to obtain 0.95 g of the extract, which was dissolved in 100 ml of water and filtered to prepare a water solution with a concentration of 0.95%. ; The indoor preventive test on cucumber downy mildew was carried out by using the leaf disk floating method, and the control effect on cucumber downy mildew reached 88.89%.
Embodiment 2
[0016] The weight percent of water agent for controlling cucumber and tomato diseases: 3% of Albizia Julibrissin leaf extract, 97% of distilled water.
[0017] Weigh 40g of Albizia Julibrissin leaf powder (20 mesh particles), soak and extract 3 times with 400ml of anhydrous methanol at room temperature, each time for 24 hours, concentrate under reduced pressure and recover the solvent to obtain 10.3g of dry matter; use ultrasonic waves to suspend the dry matter with 100ml of water, An equal volume of petroleum ether, ethyl acetate, and water-saturated n-butanol were extracted 5 times in sequence, and the n-butanol phase extract was concentrated to obtain 1.9 g of the extract, which was dissolved in 63.33 ml of water, filtered, and prepared into a 3% Water agent; the indoor pot test is used to carry out the preventive test on cucumber downy mildew, and its control effect reaches 62.00%.
Embodiment 3
[0019] The weight percent of water agent for controlling cucumber and tomato diseases: 0.95% of Albizia Julibrissin leaf extract, 99.05% of water.
[0020] Weigh 40g of Albizia Julibrissin leaf powder (20 mesh particles), soak and extract 3 times with 400ml of anhydrous methanol at room temperature, each time for 24 hours, concentrate under reduced pressure and recover the solvent to obtain 10.3g of dry matter; use ultrasonic waves to suspend the dry matter with 100ml of water, An equal volume of petroleum ether, ethyl acetate, and water-saturated n-butanol were extracted 5 times in sequence, and the n-butanol phase extract was concentrated to obtain 1.9 g of the extract. The extract was dissolved in 200 ml of water, filtered, and prepared into a water solution with a concentration of 0.95%. agent; using Tianwen district test to prevent cucumber downy mildew, its control effect reached 48.84%. The control effect is significantly different from that of the blank control (sprayi...
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