Lung cancer strain with high potential power of bone transference and its generation

A technology of lung adenocarcinoma cell line and lung adenocarcinoma cell line, which is applied in the field of lung adenocarcinoma cell line and its establishment, to achieve the effects of small cell size, high short-term tumor stagnation rate, and rapid cell growth

Inactive Publication Date: 2005-12-28
SHANGHAI CHEST HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] There is no report on the use of radioisotope bone scintigraphy to find bon

Method used

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  • Lung cancer strain with high potential power of bone transference and its generation
  • Lung cancer strain with high potential power of bone transference and its generation
  • Lung cancer strain with high potential power of bone transference and its generation

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0038] Example 1

[0039] 1. Preliminary experiment Human lung adenocarcinoma cell (SPC-A-1) 1×10 6 , Inject into the skin of immunodeficient mice. When the tumor grows to 0.5-1 cm, the tumor is taken out, and the surrounding tumor tissue is cut, mashed, and cell cultured. After the cells grow, they are expanded to the required cell volume for use.

[0040] 2. Immunodeficiency mice weigh more than 20 grams, regardless of male or female. After purchase, they will be kept in SPF cages for several days.

[0041] 3. Anesthetize experimental animals. Inject diluted thiopental sodium and ketamine into the abdominal cavity and leg muscles of the mice, respectively.

[0042] 4. Cancer cell injection: 0.7×10 was injected into the left ventricle of anesthetized mice for the first time 6 Human lung adenocarcinoma cells, SPF caged.

[0043] 5. Weighing Weigh the next day after two weeks of injection. Those weighing less than 16 grams should be closely observed to prevent death.

[0044] 6. Im...

Example Embodiment

[0048] Example 2

[0049] 1. Cellular The left ventricle of mice with immunodeficiency is injected with bone metastasis cells from the primary culture after the second cycle of cancer cells.

[0050] 2. Immunodeficiency mice weigh more than 20 grams, regardless of male or female. After purchase, they will be kept in SPF cages for several days.

[0051] 3. Tail vein injection, from the tail vein injection ≥10 6 After cancer cells, SPF cages.

[0052] 4. Weighing: Weigh every week after three weeks of injection. If the weight is less than 16 grams, observe closely to prevent death.

[0053] 5. Imaging. An isotope bone scan will be done every two weeks after one month, and the bone metastases will be taken out for in vitro culture.

[0054] 6. Subculture of bone metastasis cells in vitro Repeat the above work until a new subset of bone metastasis cells is obtained.

[0055] The results showed that 36-day radionuclide bone imaging of the fourth-generation mice injected with lung adeno...

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Abstract

Adenocarcinoma of the lung cell strain with bone transfer high potency and its construction are disclosed. The process is carried out by using human lung adenocarcinoma SPC-A-1 cell strain as source cell, immunizing deficiency mouse in vivo, externally continuously screening, image detecting from nuclein bone and constructing adenocarcinoma of the lung cell strain SPC-A-1 BM with bone transfer high potency. Its advantages include less cell volume, fast cell growth speed, better infiltration and transfer ability. It can be used for lung cancer transfer.

Description

technical field [0001] The invention belongs to the fields of biotechnology and microbial animal cell lines, and in particular relates to a lung adenocarcinoma cell line with high potential for bone metastasis and its establishment method and application. Background technique [0002] Lung cancer is one of the most common and most malignant tumors in clinical practice, and ranks among the top three in the incidence and mortality of malignant tumors in the world. In my country, especially large and medium-sized cities, lung cancer has always been the first malignant tumor killer since the 1980s. At present, the principle of clinical treatment of lung cancer is still multidisciplinary comprehensive treatment based on surgery. After decades of unremitting efforts, a considerable number of patients have achieved long-term survival by adopting early diagnosis, early treatment and active comprehensive treatment (including surgery, radiotherapy, chemotherapy and biological therapy...

Claims

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Application Information

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IPC IPC(8): C12N5/08
Inventor 扬顺芳
Owner SHANGHAI CHEST HOSPITAL
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