Method of increasing gas production rate of sewage gas fermentation using xylanase
A technology of xylanase and biogas fermentation, which can be used in fermentation, waste fuel and other directions, and can solve the problems affecting the start of biogas fermentation and the delay of biogas.
Inactive Publication Date: 2006-04-12
YUNNAN NORMAL UNIV
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Problems solved by technology
However, in our batch fermentation using pig manure as raw material, direct input of microorganisms with high xylanase activity failed to obtain the expected effect of improving gas production, and adding commercial xylanase did not achieve good results, especially And it makes the generation of biogas lag, which seriously affects the start of biogas fermentation
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Experimental program
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Effect test
Embodiment
[0037] (1) The method that xylanase is used for the start-up of new pool
[0038] Start the biogas fermentation according to the conventional biogas fermentation ingredients, and calculate the amount of xylanase added based on the actual volume of fermentation, which is 0.58IU / mL (87BU / mL). In the feeding operation, when the material is fed to 1 / 2 to 2 / 3, the required input xylanase is absorbed and dissolved with water and then poured into the pool, and then the rest of the material is used to seal the pool.
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A fermenting method using microbes and enzyme for generating marsh gas with high gas output rate features that the xylanase-type hemi-cellulase is externally added to new marsh gas pool in the ratio of 0.55-0.5 IU / ml (material liquid) and for the normal marsh gas pool, the ratio of xylanase is increased to 5.0 IU / ml. Its advantages are high gas output rate and high content of methane in gas.
Description
Technical field [0001] The invention relates to a biogas fermentation method using microorganisms or enzymes, in particular to a method in which xylanase-based cellulase is added to a biogas fermentation system from an external source to increase the gas production rate. Background technique [0002] One of the problems that always exists in rural semi-continuous and continuous biogas fermentation is the low gas production rate, and the actual raw material gas production rate is only about 50% of the theoretical value. [0003] As we all know, biogas fermentation is a process in which multi-bacteria symbiotically metabolize organic matter into methane and carbon dioxide under anaerobic conditions, that is, anaerobic digestion process, which is a unified process for processing organic waste and obtaining energy. According to different metabolites, in 1961, W.W.Eckenfelder proposed four-stage theory of hydrolysis stage, acidification stage, aci...
Claims
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IPC IPC(8): C12P5/02
CPCY02E50/343Y02E50/30
Inventor 刘士清张无敌
Owner YUNNAN NORMAL UNIV
