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Field transplant technique of pinellia tissue-culturing seedling

A technology of tissue culture seedlings and pinellia, applied in the field of field transplanting technology of pinellia tissue culture seedlings, can solve the problems of low transplanting survival rate, long cultivation time, high cost, etc., achieve high transplanting survival rate and simple implementation , the effect of easy operation

Inactive Publication Date: 2006-05-10
四川省农业科学院经济作物育种栽培研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The currently known transplanting of pinellia tissue culture seedlings mostly adopts greenhouse transplanting technology, and the investment in greenhouse facilities is high, and the transplanting survival rate is low (below 50%)
At the same time, transplanting in greenhouses requires a longer seedling age of Pinellia tissue culture seedlings, and generally requires a leaf height of 3-5 cm or more before transplanting, long cultivation time, more pests and diseases, and higher cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment 1: Pinellia tissue culture technology comprises links such as explant, culture medium, inoculation, culture condition, wherein:

[0014] Explants: Take the healthy buds of Pinellia pinellia, cultivate them on the improved MS medium, and when the buds grow to 3-4cm, take the shoot tips and cultivate them on the improved MS medium to obtain non-toxic seedlings. Take vigorously growing non-toxic seedling leaves as explants for culture.

[0015] Medium: using MS+6-BA 0.1-1.0 +2.4-D 0.1-1.0 +KT 0.1-1.0 +IAA 0.1-1.0 The culture medium was inoculated after autoclaving.

[0016] Inoculation: Divide the leaves of the above-mentioned non-toxic seedlings into inoculation materials with a diameter of 0.6-1.0mm, and inoculate them on the ultra-clean bench in the sterile room, and inoculate 15-20 pieces per bottle of culture medium. Each leaf can be cut into 50-150 small square materials depending on its size.

[0017] Cultivation conditions: After inoculation, put t...

Embodiment 2

[0024] Embodiment 2: the field transplanting technique of pinellia tissue culture seedlings comprises slurry box transplanting, cover soil, sunshade, field management steps, wherein:

[0025] Transplanting in slurry box: apply 3000kg of manure or compost per mu of seedbed, deep plowing, after 20 days in the sun, then deep plowing again, thinning and raking, start a box with a width of 1m and a box height of 5cm. Then sprinkle water on the box, soak the whole surface of the box with clean water to a depth of about 15cm, if there is excess water on the surface of the box, smooth the surface of the box, keep the surface of the box smooth and let the box dry for half a day for later use;

[0026] When the Pinellia tissue cultured seedlings have small buds growing or have buds or seedlings, or sprouting small buds, and the length is greater than 0.5cm, they can be taken out for transplanting; after the tissue cultured seedlings are taken out from the cultivation room, practice seedl...

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PUM

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Abstract

The invention discloses a field replanting technique for a pinellia tuber tissue culture sprout, comprising: plasm replanting, covering soil, sunlight shading and field care; comparing with the current technique the invention is characterized by the high survival rate of the pinellia tuber tissue culture sprout, reaching above 95%, low cost, fast growing, simple implement of the technique and operation.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to the field transplanting technology of Pinellia tissue culture seedlings, the transplanting survival rate of this transplanting technology can reach more than 90%, the age of transplanted seedlings is advanced, and the transplanting growth it is good. Background technique [0002] Pinellia is the tuber of Araceae, Pinellia, and plant Pinellia. Because it begins to germinate and grow leaves in the fifth month of the lunar calendar, when the Lixia season has passed, it is named "Ban Xia". Pinellia is a commonly used traditional Chinese medicinal material. Its functions are to reduce phlegm, harmonize the stomach, and relieve vomiting. It can treat cough, phlegm stagnation, wheezing, nausea and vomiting. It contains β-sitosterol-D-glucoside, black uric acid (homogentisic acid) and aspartic acid and other amino acids and eighteen kinds of trace eleme...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G7/00
Inventor 胡尚钦黄璐琳杨晓江怀仲
Owner 四川省农业科学院经济作物育种栽培研究所