Healthy polysaccharide food come from sea invertebrate

A technology for invertebrates and health food, applied in the field of polysaccharide health food and its preparation, to achieve the effects of eliminating heavy metals, lowering cholesterol, and promoting immune function

Active Publication Date: 2006-05-10
DALIAN GLYCOBIO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The health food of polysaccharides from

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Wash at 0°C; cook at 70°C for 10 minutes under 0.3MPa pressure, filter, and collect the cell extract; centrifuge to remove the precipitate, adjust the cell extract to pH 5 with 70% acetic acid, and acidify; Separation method Extraction, the membrane separator cuts off substances with a molecular weight of 10000Da or less, adjusts the pH of the acid solution to 6 with NaOH; centrifuges to remove precipitation, dialyzes in distilled water to remove salt, adopts the membrane separation method, the membrane separator cuts off the molecular weight It is 500-1000 Da; freeze-dried to obtain marine invertebrate mussel polysaccharide.

[0015] The detected composition and structure of the polysaccharide are: 80-85% α-1,4; 1,6-glucan and β-1,3-glucan, and the relative molecular weight is 4000-6000Da. 15-20% galactose-specific mussel sugar-binding protein. The carbohydrate-binding protein has a molecular weight of 15-25 kDa.

Embodiment 2

[0017] Wash at 10°C; cook at 80°C for 7 minutes under 0.5MPa pressure, filter, and collect the cell extract; centrifuge to remove the precipitate, adjust the cell extract to pH 6 with 60% acetic acid, and acidify; Separation method Extraction, the membrane separator cuts off substances with a molecular weight of 10000Da or less, adjusts the pH of the acid solution to 8 with NaOH; centrifuges to remove precipitation, dialyzes in distilled water to remove salt, adopts the membrane separation method, the membrane separator cuts off the molecular weight It is 500-1000 Da; freeze-dried to obtain marine invertebrate mussel polysaccharide.

Embodiment 3

[0019] Wash at 4°C; cook at 75°C for 8 minutes under 0.4MPa pressure, filter, and collect the cell extract; centrifuge to remove the precipitate, adjust the cell extract to pH 6 with 65% acetic acid, and acidify; Separation method Extraction, the membrane separator cuts off substances with a molecular weight of 10000Da or less, adjusts the pH of the acid solution to 7 with NaOH; centrifuges to remove precipitation, dialyzes in distilled water to remove salt, adopts the membrane separation method, the membrane separator cuts off the molecular weight It is 500-1000 Da; freeze-dried to obtain marine invertebrate mussel polysaccharide.

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Abstract

The invention provides a polysaccharide health products from ocean invertebrate, rear case mussel, mussel and jade mussel, prepared by following steps: washing at temperature of 0-10 Deg, C; boiling and filtering at pressure of 0.3-0.5 MPa, and temperature 70-80 Deg, C, collecting cell extract; centrifuging to remove deposition, moderating the pH of cell extract y to 5-6 with acetic acid with the concentration being 60-70%, acid splitting; extracting with membrane separating process, the substance entrapped by membrane segregator is that with the molecular weight being 10000 Da or lower than 10000 Da, moderating the pH of the acid hydrolysis solution with NaOH to 6-8; centrifuging to remove deposition, dialyzing to remove salt in distilled water; employing membrane separating process, with the molecular weight entrapped by membrane segregator being 500-1000 Da; freezing and drying, and getting mussel polysaccharide from ocean invertebrate. The product is characterized by the functions of reducing cholesterol, increasing blood content, resisting radiation and removing heavy metals.

Description

technical field [0001] The invention relates to health food, in particular to a polysaccharide health food from marine invertebrates and its preparation. Background technique [0002] In recent years, with the gradual recognition of radiation pollution, people urgently need to find a radiation-resistant active substance. The polysaccharides found in the ocean have been proved by animal experiments to have good anti-radiation activity and can be used as anti-radiation health food. [0003] Marine organisms such as kelp and wakame contain a large amount of specific polysaccharides. This kind of polysaccharide has many uses, such as carrageenan can be used in pharmaceutical matrix, can resist thrombosis; seaweed polysaccharide can excrete heavy metals in the body; fucoidan has anti-tumor effect. The health food of marine animal polysaccharides has not been reported yet. Contents of the invention [0004] The object of the present invention is to provide a kind of polysacch...

Claims

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Application Information

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IPC IPC(8): A23L33/10
Inventor 李伟赵小明李曙光白雪芳曲天明杜昱光
Owner DALIAN GLYCOBIO
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