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Zushi daphneol extract, its preparation method and use

A technology of alcohol extract and extract, which is applied in the direction of drug combination, pharmaceutical formula, plant raw materials, etc., can solve the problems of high price and large side effects

Active Publication Date: 2006-06-14
辉源生物科技(上海)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, statins have relatively large side effects and are expensive, so there is an urgent need for new cholesterol-lowering drugs with less toxic side effects

Method used

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  • Zushi daphneol extract, its preparation method and use
  • Zushi daphneol extract, its preparation method and use
  • Zushi daphneol extract, its preparation method and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The preparation of embodiment 1 Zuma alcohol extract

[0041] 1. Experimental equipment and reagents:

[0042] Pulverizer (Beijing Guangming Instrument Factory FZ-102) Isotope bottle

[0043] Rotary evaporator (Shanghai Shensheng Instrument Co., Ltd.) Four-hole water bath (Beijing Instrument Factory)

[0044] 95% ethanol (analytical grade, Shanghai Reagent No. 1 Factory) large beaker

[0045] Electronic balance (accurate 0.1g, Beijing Medical Balance Factory DT-500) experimental filter paper

[0046] TP300 Ultrasonic Cleaner (Beijing Tianpeng Electronic Technology Co., Ltd.)

[0047] (The relevant instrument models mentioned below are no longer indicated)

[0048] 2. Source of experimental medicinal materials:

[0049] It was purchased in Anguo, Anhui in November 2001. It was identified by Professor Yan Yuning of Beijing University of Traditional Chinese Medicine and put into storage with a serial number.

[0050] Identification results: stem bark and root bark of D...

Embodiment 2

[0058] The liquid phase separation of embodiment 2 Zuma alcohol extract

[0059] 1. Experimental equipment

[0060] HPLC preparation system (Shimadazu Class_vp HPLC, Shimadazu Instrument Analysis Institute, Japan)

[0061] Methanol (HPLC grade, 4L, Fisher)

[0062] h 2 O (Millipore 0.22um filter)

[0063] Chromatographic column (YWG C18 10u 250×10, Beijing shuntu biotech.co., LTD)

[0064] 2. Experimental steps:

[0065] (1) After shaking the ethanol extract of Zumima (approximately liquid) obtained in Example 1, take 200ul respectively in four eppendorfs (1.5ml, domestic). And take 800ul of pure methanol in these four eppendorf tubes. Shake well to dissolve it fully. Centrifuge and filter. Take the supernatant in a 4ml injection bottle. Take out 1ml of the supernatant, centrifuge and concentrate, and calculate the concentration of the supernatant after drying (to ensure reproducibility, this configuration method is almost the same as the first preparation, and the fi...

Embodiment 3

[0074] Example 3 Zumima alcohol extract reduces density lipoprotein activity test

[0075] 1. Materials and Methods

[0076] Drugs to be tested: the ethanol extract of Zumima obtained in Example 1, and the F15 and F16 component samples obtained in Example 2.

[0077] Plasmid: pLDLR270-luc was constructed by Shanghai Huada Tianyuan Biotechnology Co., Ltd., and the LDLR promoter region cloned from the genome was inserted into the pGL3-basic vector (Promega, USA).

[0078] Cells: pLDLR270-luc stably transfected human embryonic kidney cells (HEK 293) (CRL-1573), human liver cancer cells HepG2 (ATCC HB-8065).

[0079] Reagents: KpnI, BglII (NewEngland Biolab), pGL3-basic vector (Promega, USA), T4 ligase (Promega, USA), Lipofeetamine2000 (Invitrogen, USA), DMEM medium (GIBCO), streptomycin, L-Glutamine (Invitrogen, USA), Hygromycin (Roche, Germany), Fetal Bovine Serum (Hyclone, USA), Heparin (Hepes), Luciferase Assay System and Bright-Glo Luciferase Assay System (Promega, USA), R...

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Abstract

An application of giraldi daphne bark and its extract in preparing the medicines for lowering the level of low-density lipoprotein and preventing and treating the relative diseases is disclosed. Said extract is prepared through immersing giraldi daphne bark in alcohol, and filtering to obtain filtrate, and can also be used as the promotor for the cell surface acceptor expression of low-density lipoprotein.

Description

technical field [0001] The invention relates to the field of traditional Chinese medicine, in particular to an alcohol extract of zushima and its application. The present invention also relates to the composition containing the zumi alcohol extract. Background technique [0002] Zushi Ma is the root bark and stem bark of Dolphne giradii Nitsche, a plant belonging to the genus Daphne in the family Thymelaeaceae. The same plants of the same genus Daphne tangutica Maxim, Daphne retusa Hemsl, and Daphne changbai D. koreana Nakai can be used as medicine. Zushima has the effects of expelling wind and dampness, relieving pain and dissipating stasis, and is widely used in folks to treat pain, bruises, rheumatoid arthritis and bronchitis. [0003] Chinese Journal of Traditional Chinese Medicine, Vol. 27, No. 6, June 2002. The review describes the research progress of some chemical constituents and pharmacological activities in Zushima. It discloses that the chemical components in z...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/83A61K125/00A61K135/00A61P3/06A61P3/10A61P9/10
Inventor 张以琳张海青华仕能姜立群郭海燕谈学海
Owner 辉源生物科技(上海)有限公司