Pyronone antibiotic its preparation method and application

A technology of pyrone and antibiotic, which is applied in the field of pyrone antibiotic and its preparation, and can solve the problems of no preparation method and agricultural preparation, etc.

Inactive Publication Date: 2006-06-14
BIOTECH CENT OF SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Trichoderma is an important plant disease biological control bacteria, which can produce a variety of antibiotics, among which pyrone compounds have been proved to have strong antibacterial activity, but there is no specific preparation method and agricultural preparation

Method used

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  • Pyronone antibiotic its preparation method and application
  • Pyronone antibiotic its preparation method and application
  • Pyronone antibiotic its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Embodiment 1: the solid fermentation culture of Trichoderma viride LTR-2, the following are weight ratios or weight percentages.

[0053] Trichoderma viride LTR-2, the preservation number is CGMCC NO.1498, is isolated and screened from soil, and it is found through indoor plate antagonism experiments and field biological control experiments that the strain has a wide antibacterial spectrum, good control effect, and is easy to cultivate , no pollution.

[0054] The solid fermentation culture of Trichoderma viride LTR-2 is carried out in the following steps:

[0055] (1) Incline strains: using solid PDA medium, the Trichoderma viride LTR-2 was inoculated on the test tube medium, and cultured at 28° C. for 2-3 days.

[0056] (2) Strains in eggplant bottles: using liquid PDA medium, inoculate the strains in test tubes into liquid eggplant bottles, and place them on a shaker at 28° C. for shaking culture for 3-4 days.

[0057] (3) Liquid strain: use seed culture medium, co...

Embodiment 2

[0061] Embodiment 2: the preparation of pyrone antibiotics, the following are weight percentages.

[0062] (1) Spore preparation

[0063] The solid culture of Trichoderma viride LTR-2 (the product of Example 1) was sieved with a 100-mesh sieve to collect the spore powder.

[0064] (2) Extraction of pyrone antibiotics in spores

[0065] Add 50g / 1L of spore powder into dichloromethane and soak at 5°C for 2 days, absorb the soaking solution with 5% activated carbon for 2 hours, filter through filter paper to remove activated carbon and spore residue, add an equal volume of 3% sodium carbonate solution to the supernatant and wash , repeat once, after combining the sodium carbonate solutions, wash with a little dichloromethane, incorporate into the dichloromethane soaking solution, then add anhydrous sodium sulfate for dehydration, and concentrate the dichloromethane soaking solution under reduced pressure at 35°C until viscous liquid to obtain a concentrate containing pyrone ant...

Embodiment 3

[0074] Embodiment 3: the agricultural formulation of antibiotic pyrone, all in parts by weight:

[0075] Diatomaceous earth 84 parts

[0076] Concentrate containing pyrone 10 parts

[0077] Sodium dodecylbenzenesulfonate 5 parts

[0078] Tween 80 1 serving.

[0079] The above-mentioned pyrone-containing concentrate is the product of step (2) in Example 2.

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Abstract

The present invention relates to a pyrone antibiotic, its preparation method and application. Its molecular formula is C10H16O2, and its molecular weight is 168. Said invention also provides its molecular structure diagram. Its preparation method includes the following steps: using solid culture medium to culture green trichoderma LTR-2, obtaining spore powder; soaking spore powder in dichloromethane with 5-10deg.C, making the soaking liquor successively undergo the processes of adsorption with active carbon, washing by using sodium sulfate solution, dehydration by using anhydrous sodium sulfate and reduced pressure concentration to obtain concentrate containing antibiotic pyrone. It can be used as effective component to prepare an agricultural preparation-wettability powder preparation whose composition includes diatomite, concentrate containing pyrone, sodium dodecyl benzene sulfonate and Tween 80.

Description

technical field [0001] The invention relates to pyrone antibiotics and a preparation method and application thereof, belonging to the field of biotechnology. Background technique [0002] Trichoderma (Trichoderma spp.) has an advantage in producing antibiotics. As far as its species are concerned, there are only about 70 kinds of antifungal metabolites, and most species produce more than one kind of antibiotics. For example, Trichoderma harzianum can produce 12 kinds, Kang Trichoderma can produce 9 species, Trichoderma viride can produce 10 species, Trichoderma hacinata can produce 7 species, Trichoderma longibrachiae can produce 3 species, and Trichoderma multispora can produce 2 species. [0003] Dennis, Webster, Non-volatile antibiotics produced by Trichoderma, Mycology, 1971, No. 57 (DENNIS C, WEBSTER J.Antagonistic properties of species-groups of Trichoderma production of non-volatile antibiotics[J].Trans Br Mycelia Soc, 1971, 57:25-39.) It is believed that antibacteri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D309/38A01N43/16
Inventor 杨合同陈凯王加宁李纪顺扈进冬张广志
Owner BIOTECH CENT OF SHANDONG ACAD OF SCI
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