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Isolamento e uso de fungos deteriorados

A decay and fungus technology, applied in the field of separation and application of decay bacteria, which can solve problems such as high cost, engineering requirements and logistical needs

Inactive Publication Date: 2006-07-26
UNIVERSITY OF WAIKATO +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although decay fungi have been reported to be used for delignification of wood (see Erickson, K.E. and Kirk, T.K. "Biopulping, biobleaching and treatment of kraft bleaching effluents with white-rotfungi". In: Comprehensive Biotechnology: the principles, applications and regulations of biotechnology in industry, agriculture and medicine. Pergamon Press, New York. 271-294. (1985); Eriksson (1992); Blanchette R.A. et al., US 5,476,790; Blanchette R.A. et al., US 5,705,383; Gonzalez M.J. et al., "Biopulping for Kraft Pulp of Pinus radiata". Actas50th Conference. APPITA, V, (1996); González M.J. et al., "Refining biokrafi pulp of Radiata pine". APPITA General Conference. (1997); Gonzalez M.J. et al., "Biopulp from radiata Pine". Actas 10th International Symposium on Wood and Pulping Chemistry. Yokohama, Japan, 06 (1999); González M.J. et al., "Yieldincrease with softwood Kraft Biopulp". TAPPI Pulping / process & Product Quality Conference. Boston, USA. (2000); Donoso J. et al., "Influence of ecological factors in the behavior of White Decay Fungi". Actas 53 APPITA Annual Conference. R otorua, New Zealand, 03 (1999)), this technology has not been practically used commercially, mainly because of the unmatched characteristics of the desired decaying bacteria and the unacceptably high level of degradation of cellulose by said fungi
It thus has commercial limitations because applying the technology to wood as a substrate, whether round logs, wood chips, or veneers, requires decontamination prior to fungal inoculation and provision of nutrients to stimulate fungal growth, typically requiring atmospheric steam processing , to obtain sterile, or in some cases nearly sterile (i.e. decontaminated) substrates
This creates higher costs, engineering requirements and logistical needs

Method used

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  • Isolamento e uso de fungos deteriorados
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  • Isolamento e uso de fungos deteriorados

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0132] A non-limiting application is described below, which describes an example procedure for obtaining and culturing the spoilage fungus of the present invention.

[0133] The procedure includes the following steps:

[0134] a) Collection of fungi in nature, wherein fungal material is isolated from infected lignocellulosic matter in the form of sexual bodies. Said fungi are then identified in the laboratory by using appropriate taxonomic means;

[0135] b) Isolating and culturing said spoilage bacteria in a growth medium. For example a mixture of agar and malt extract, more specifically 1% to 3% agar and 2% to 15% malt extract is used as a growth medium and may be supplemented with antibiotics such as penicillin and streptomycin;

[0136] c) determine the presence of oxidase by any standard method (such as the Nobleman method);

[0137] d) determining the antimicrobial effect exhibited by said fungus;

[0138] e) preparing a nutrient support for fungal propagation;

[0...

Embodiment 2

[0141] A non-limiting example of the invention is described below to illustrate the steps of the inventive method of the invention, the results of applying decay bacteria during delignification of papermaking wood, and the results of the end product of the pulping process.

[0142] a) Isolation: Blocks with decaying bacteria are mainly placed in agar-malt cultures supplemented with bacterial antibiotics, such as figure 1 explained in. Once the mycelium is formed the fungus is purified using standard microbiological techniques.

[0143] b) Prepare viable forms and quantities of decaying bacteria that can reproduce in solid wood residues: such as figure 2 As shown, the selected decaying bacteria were inoculated on wood residues such as wood chips, wood chips of forest species and / or sawdust with a moisture content of 60% to 80% in a natural state or after disinfection treatment. Down will be treated in a pre-delignification process.

[0144] c) Determination of the antimicro...

Embodiment 3

[0161] The following example presents data obtained in an attempt to apply decay bacteria on radiata pine pulp in order to determine the bleaching capacity of said decay bacteria. Delignification of radiata pine wood treated with the decay bacteria prior to kraft pulping was also observed.

[0162] Unbleached radiata pine pulp with kappa numbers 28, 32, 35 and 42 was treated with two decay fungus strains (9C and 24P). The treatment was carried out under the condition that the dry weight of decaying bacteria was 0.06% of the dry weight of pulp, and the pulp was maintained at a pH of 6 and a temperature of 25°C. Decay bacteria are applied to the pulp at the Bristol-board stage, which is then broken down and brought back to a moisture content of 70%. After the treatment is complete, the pulp is disintegrated and produced approximately 60g / m 2 paper in order to determine the paper processing properties. Kappa number, viscosity, rupture length, tear index, brightness and opacity...

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Abstract

The invention relates to methods for the isolation and development of fungi useful in the enhancement of wood or wood products quality and / or wood or wood products processing, and the decay fungi so developed. Compositions comprising or including decay fungi and methods for the use of said decay fungi and / or compositions are also provided. The decay fungi, compositions and methods are useful to provide improved cellulosic pulp production and / or wood and / or wood products of enhanced quality from non-sterilized wood.

Description

technical field [0001] The present invention relates to the cultivation of fungi useful for enhancing the quality and / or processing of wood or wood products. More particularly but not exclusively, the present invention relates to methods for isolating and cultivating decay fungi, compositions comprising or comprising decay fungi, and methods of application of said decay fungi and / or compositions, said decay fungi The compositions and methods are useful for providing improved cellulose pulp production from non-sterilized wood and / or wood and / or wood products of better quality. Background technique [0002] The pulp industry is based on the separation of fibres, and most pulp in the world is mainly produced chemically by digestion. An example is a kraft system using wood as raw material, either from natural forests or plantations, such as Pinus radiata and Eucalyptus spp. The purpose of this digestion process is to reduce or eliminate lignin to obtain cellulose pulp. [000...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01G1/04A01H15/00D21C1/00D21C3/02D21C5/00
CPCD21C1/00D21C3/02C12R1/645D21C5/005C12N1/14C12R2001/645C12N1/145
Inventor 罗伯塔·李·法雷尔胡安·哈维尔·冈萨雷斯·莫利纳胡安·爱德华多·多诺索·古铁雷斯
Owner UNIVERSITY OF WAIKATO