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Culture medium for anthurium andraeanum tissue culture and tissue culture seedling breeding method

A technology of anthurium group and culture medium, which is applied in the field of tissue culture seedling propagation, can solve the problems of immature reproduction technology of regenerated plants, limit the practical application of research results, and have not yet seen callus induction and plant regeneration, etc., so as to promote The effect of propagation speed, mitigation of impact, and promotion of callus proliferation

Inactive Publication Date: 2006-08-09
BEIJING NORMAL UNIVERSITY +1
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AI Technical Summary

Problems solved by technology

[0006] So far, there have been no reports about a basic medium suitable for callus induction and plant regeneration of two Anthurium species
[0007] (2) The varieties or genotypes that can be reproduced by the existing technology are limited
These research reports indicate that the existing medium can only induce callus and regenerate plants of individual varieties, and no medium for callus induction and plant regeneration independent of Anthurium varieties or genotypes has been developed.
[0009] (3) The reproductive technology of potted seedling leaves and petiole explant regeneration plants of vegetative propagation is immature
Existing research has only been successful on individual varieties, which limits the practical application of research results

Method used

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Embodiment Construction

[0050] Firstly, the culture medium required for propagating Anthurium tissue culture seedlings is prepared, and the preparation of the culture medium adopts a conventional culture medium preparation method. Callus induction and adventitious bud differentiation medium adopt induction medium, the formula of this induction medium is:

[0051] Recipe 1:

[0052] Macroelement Composition: KNO 3 , 600mg / L; NH 4 NO 3 , 200mg / L; CaCl 2 2H 2 O, 220mg / L; MgSO 4 ·7H 2 O, 370mg / L; KH 2 PO 4 , 170mg / L;

[0053] Trace element composition: FeSO 4 ·7H 2 O, 13.9 mg / L; Na 2 EDTA·2H 2 O, 18.65mg / L; KI, 0.83mg / L; H 3 BO 3 , 6.2mg / L; MnSO 4 4H 2 O, 22.3mg / L; ZnSO 4 ·7H 2 O, 8.6 mg / L; Na 2 MoO 4 2H 2 O, 0.25mg / L; CuSO 4 ·5H 2 O, 0.025mg / L; CoCl 2 ·6H 2 O, 0.025mg / L;

[0054] Organic nutrients: inositol, 1000mg / L; niacin, 0.5mg / L; pyridoxine hydrochloride, 0.5mg / L; thiamine hydrochloride, 0.1mg / L; glycine, 2.0mg / L;

[0055] Growth regulator: 2,4-dichlorophenoxyacetic acid...

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Abstract

The present invention discloses a culture medium for tissue culture of Anzuhua flower and its propagation method by using tissue culture seedlings. Said culture medium is characterized by that it contains macro-element components and abscisic acid, and its propagation method includes the following steps: utilizing the above-mentioned culture medium to induce leaf or petiole to produce callus and adventitious bud; successively transferring the callus whose adventitious bud is differentiated to improved MS culture medium to make culture and propagate adventitious bud; transferring the adventitious bud into rooting culture medium and inducing it to root, then using conventional tissue culture seedling transplantation method to transplant the rooted tissue culture seedling into soil matrix.

Description

technical field [0001] The invention relates to a tissue culture seedling (test-tube seedling) propagation technology in plant biotechnology, in particular to a culture medium for tissue culture of Anthurium anthurium and a method for propagating the tissue culture seedling. Background technique [0002] Anthurium (also known as Anthurium, Anthurium, Anthurium) is a plant of the genus Anthurium in the family Araceae. The main commercial flower cultivars are A.andreanum and A.scherzerianum, whose sales volume ranks second in the world flower market, and they are rare flowers second only to tropical orchids. The propagation of Anthurium seedlings is mainly propagated by the method of tissue culture seedling cultivation. Since Pierik et al. (1974) induced A.andreanum to form callus, Pierik (1976), Kunisaki (1980), Kuehnle and Sugii (1991), Teng (1997), Chen et al. (2000), Joseph et al. (2003), Martin et al. (2003), Vargas et al. (2004) and Guo Weiming et al. (2004) reported t...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 肖尊安
Owner BEIJING NORMAL UNIVERSITY
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