Electrochemical biosensor

A biosensor, electrochemical technology, applied in the direction of scientific instruments, biological testing, biochemical equipment and methods, etc.

Active Publication Date: 2006-08-09
ISENSE CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is almost impossible to achieve this desired end using hitherto known techniques

Method used

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  • Electrochemical biosensor
  • Electrochemical biosensor
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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0118] Example 1: Preparation of a fatty acid-free reagent layer composition solution

[0119] Will contain 30mg hexaamine ruthenium (III) chloride (41.6% by weight), 1mg carboxymethyl cellulose (1.4% by weight), 1mg Triton X-100 (14% by weight) and 40mg glucose oxidase (55.6% by weight) The mixture was dissolved in 1 ml PBS buffer (pH 6.4), and then undissolved particles were filtered out. Place the reagent solution thus obtained in the syringe of a pneumatic dispenser (EFD XL100).

Embodiment 2

[0120] Example 2: Preparation of a reagent layer composition solution containing fatty acids

[0121] Will contain 30mg hexaamine ruthenium (III) chloride (41.6% by weight), 1mg carboxymethyl cellulose (1.4% by weight), 5mg polyvinylpyrrolidone (4% by weight), 1mg Triton X-100 (1.4% by weight), A mixture of 20 mg lauric acid (15.7 wt%), 30 mg myristyl trimethylammonium bromide (23.6 wt%) and 40 mg glucose oxidase (55.6 wt%) was dissolved in 1 ml PBS buffer (pH 6.4), and then filtered Remove undissolved particles. The reagent solution thus obtained was placed in the syringe of a pneumatic dispenser (EFDXL100).

Embodiment 3

[0123] In this embodiment, a method of measuring blood glucose level is described. use figure 2 with 3b The illustrated auxiliary electrode 105 serves as a reference electrode in a thin-layer electrochemical cell type biosensor. The following method is used to manufacture a thin-layer electrochemical cell for measuring blood glucose level.

[0124] Such as figure 2 , 3a As shown in and 3b, the working electrode 104 and the electrode connector 106, which are thick enough to be three-dimensionally connected to the auxiliary electrode, are screen-printed with conductive carbon paste, and then cured at 140°C for 5 minutes. Next, silver paste for circuit connectors is screen printed on one end of the electrode connector 106 to the thickness of the middle substrate 200. Likewise, the reference (auxiliary) electrode 105 is screen-printed on the upper substrate 300 with carbon paste and cured under the same conditions as the electrode of the lower substrate 400. Finally, silver paste is...

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PUM

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Abstract

Disclosed herein is a method for measuring blood glucose levels, using an electrochemical biosensor provided with a converse-type thin-layer electrochemical cell. The electrochemical cell comprising: a working electrode formed on a flat insulating substrate; an auxiliary electrode formed on a separate flat insulating substrate so as to face the working electrode; a fluidity-determining electrode, formed at a predetermined distance from the working electrode on the flat insulating substrate used for the working electrode or the auxiliary electrode; an adhesive spacer, provided with a sample-introducing part having a micro-passage, for spatially separating the working electrode and the auxiliary electrode by being interposed therebetween; an electrode connector, printed with a thick conductive material on a portion of the auxiliary electrode, for three-dimensionally connecting the working electrode to the auxiliary electrode; and a reagent layer containing an electron transfer mediator and an oxidation enzyme.

Description

Technical field [0001] The present invention relates to a method for measuring blood glucose level using an electrochemical biosensor. Background technique [0002] In order to diagnose and prevent diabetes, the importance of regular monitoring of blood glucose levels has been increasingly emphasized. Nowadays, strip biosensors designed for handheld reading devices are usually based on colorimetric or electrochemical methods, allowing individuals to easily monitor the glucose level in the blood. [0003] The following reaction formula I explains the electrochemistry applied to biosensors, and the use of electron transfer mediators is its characteristic. Examples of electron transfer mediators include: ferrocene and its derivatives; quinine and its derivatives; organic or inorganic compounds containing transition metals, such as hexaamine ruthenium, osmium-containing polymers, potassium ferricyanide, etc.; organic conductivity Salt; and viologen. [0004] Reaction formula I [000...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/66G01N27/403
CPCC12Q1/006G01N27/3272G01N33/49
Inventor 崔刚俞在炫金文焕金周勇李宗洙金槿起南学铉车根植
Owner ISENSE CORP
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