Methods and compositions for controlling ectoparasites

A technology of ectoparasites and compounds is applied in the fields of inhibiting the hatching of ectoparasite eggs, treating and preventing ectoparasite infection, identifying compounds that can inhibit the hatching of ectoparasite eggs, and can solve the problems of inconvenience and increasing the danger of environmental hosts.

Active Publication Date: 2006-08-23
HATCHTECH PTY LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Methods and compositions for controlling ectoparasites
  • Methods and compositions for controlling ectoparasites
  • Methods and compositions for controlling ectoparasites

Examples

Experimental program
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Effect test

Embodiment 1

[0188] Example 1-Evaluation of the hatching mechanism of lice eggs:

[0189] Evaluate the mechanism of lice egg hatching under a dissecting microscope. The female clothes lice were fed on the rabbit for half an hour and then transferred to a petri dish with human hair. The petri dish was then placed in an incubator at 32°C and 42% relative humidity. During the 5-hour feeding period, female lice begin to lay eggs. Each female lice lays up to 10 eggs in one hatch. The eggs develop in the next 7-9 days. The following changes are observed in the best 12 hours before hatching. With the developing embryo oriented with its head close to the hatching flap or lid, the eye of the developing embryo inside the egg is clearly detected. The embryo is observed to move inside the egg. Hatching occurs when the lid is opened, after which the embryos emerge quickly. First expose the head, then connect the chest to the legs. Finally, the abdomen protrudes and the larva crawls out of the egg, the egg ...

Embodiment 2

[0190] Example 2-Detection of protease activity in lice egg extract:

[0191] Within 12 hours of incubation, 50 individual lice eggs (Pediculus hunzcarauslaunzanus) were removed from the hair and placed in a 1ml eppendorf tube. 20 μL of distilled water was added to the unhatched eggs, and incubated at 32°C for 30 minutes. 20 μL was recovered and stored at -70°C. Many other samples were collected as described above. And collect the hair samples from which the unhatched eggs have been removed, and incubate according to the above steps. In addition, samples of unhatched eggs 7 days after laying eggs (within 24 hours of egg hatching) and hair samples from which lice eggs were removed were collected. Both samples were washed with 10 ml of 1% sodium hypochlorite solution for 1 minute, followed by washing with 25 ml of distilled water for 5×1 minutes to remove hypochlorite. Then, these samples were incubated in 20 μL of distilled water as described above. In addition, a group of 25 lice ...

Embodiment 3

[0195] Example 3: Characteristics of protease in eggshell washing:

[0196] In order to evaluate the possibility of lice hatching protease in eggshell washing as a target to inhibit egg hatching, the characteristics of hatching protease must first be determined. Four protease inhibitors are used to classify the proteases in ESW.

[0197] The 10% SDS-PAGE gelatin substrate gel was loaded in a freeze-dried egg shell wash from 100 lice eggs, which had been resuspended in 50 μl non-reducing sample buffer, and the sample was run at 10 μl / channel. The gel was run at 10 mA / gel at 4°C for 10 minutes, followed by 15 mA / gel for another 25 minutes. Then, the gel was cut into bands, and each band was incubated in a 2.5% Triton-X 100 solution containing a specific inhibitor for 2×20 minutes. The inhibitors used were serine protease inhibitor PMSF (5mM), metalloproteinase inhibitor 1,10-phenanthroline (10mM), aspartic protease pepstatin (5plM) and cysteine ​​inhibitor E-64 (10mM). Then, the gel...

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Abstract

A method for inhibiting hatching of an ectoparasite egg, the method comprising exposing the ectoparasite egg to at least one metal chelating agent and/or metalloprotease inhibitor, wherein the metal chelating agent is a compound comprising at least two heteroatoms able to simultaneously coordinate with a metal ion, at least one of the two heteroatoms being selected from nitrogen, sulfur, oxygen and phosphorus, wherein the compound comprises at least one carbocyclic ring substituted with at least one heteroatom and/or with a substituent containing at least one heteroatom, or the compound comprises at least one heterocyclic ring containing at least one heteroatom, wherein said heterocyclic ring is optionally substituted with at least one heteroatom and/or with a substituent containing at least one heteroatom is provided. Methods of treating ectoparasite infestations and compositions for use in such methods are also provided.

Description

Invention field [0001] The present invention relates to methods and compositions for controlling ectoparasites. Specifically, the present invention relates to methods and compositions for inhibiting hatching of ectoparasite eggs. The present invention also provides methods and compositions for treating and preventing ectoparasite infections. The invention also relates to methods of identifying compounds that inhibit the hatching of ectoparasite eggs. Background technique [0002] Ectoparasites, including certain insects, cause significant pest problems in many types of animals and plants. In particular, ectoparasites usually harass, bite people and livestock and cause infections. Of particular concern are the presence of these parasites and their impact on people, household pets or companion animals such as dogs and cats, and other domesticated animals such as horses. [0003] Various compositions and application methods are known for controlling or eliminating biting and blood-s...

Claims

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Application Information

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IPC IPC(8): A61K38/55A61K38/04A61K31/444A61K31/4015A61P33/14
Inventor V·M·鲍尔斯
Owner HATCHTECH PTY LTD
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