Method for separating and purifying fatty acid ester of lutein in high content from resin of marigold oil
A fatty acid ester and lutein technology, applied in the field of phytochemistry, can solve the problems of unfavorable industrial production, residue, high solvent, etc., and achieve the effect of easy large-scale industrial production, suitable for large-scale industrial production, and high biological activity
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Embodiment 1
[0036] 1000g marigold oleoresin (total lutein fatty acid ester content is 31.8%) is mixed with the mixed solvent that 5000ml edible grade dehydrated alcohol and 3000ml n-hexane are formed, is heated up to 80 ℃ and refluxes and stirs, until forming homogeneous solution, here Keep warm at high temperature for 3 hours, then slowly lower the temperature of the solution to 25°C within 2.5 hours, keep warm and crystallize, and separate the obtained crystals with a centrifuge to filter and separate the obtained crystals. The fatty acid ester content is 71% (weight percentage content). The sample was saponified and tested by HPLC, wherein all-trans lutein accounted for 92%, zeaxanthin accounted for 7%, and the rest were a small amount of cis-isomers and a small amount of other carotenoids.
[0037] Mix 400.0g of intermediate crystals obtained with 1000ml of n-hexane, heat up to 70°C and reflux for 2.0 hours under stirring, then slowly cool down to 10°C to crystallize, slowly add 80ml ...
Embodiment 2
[0041] 1000g marigold oleoresin (total lutein fatty acid ester content is 22.7%, wherein all-trans lutein ratio is 72.7%) mixes with the mixed solvent that 5600ml edible grade dehydrated alcohol and 2000ml normal hexane form, is warming up to 80 Reflux and stir at ℃ until a homogeneous solution is formed, keep at this temperature for 5.0hr, then slowly lower the solution temperature to 30℃ within 2.0 hours, keep warm and crystallize, and separate the obtained crystal by pressure filtration method, the obtained intermediate quality is 392.5g, detected by UV, the content of lutein fatty acid ester in this crystalline solid is 68.5% (weight percentage content). The sample was saponified and tested by HPLC, wherein all-trans lutein accounted for 90.5%, zeaxanthin accounted for 8.3%, and the rest were a small amount of cis-isomers and a small amount of other carotenoids.
[0042] Mix 390.0g of intermediate crystals obtained with 1500ml of n-hexane, and heat up to 70°C under stirrin...
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