Method for separating and purifying fatty acid ester of lutein in high content from resin of marigold oil

A fatty acid ester and lutein technology, applied in the field of phytochemistry, can solve the problems of unfavorable industrial production, residue, high solvent, etc., and achieve the effect of easy large-scale industrial production, suitable for large-scale industrial production, and high biological activity

Active Publication Date: 2006-12-06
浙江可明生物医药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0012] Thereby, there is following shortcoming in above-mentioned method, for example: the yield of product is not high, and purity is not enough; Large, unfavorable to industrial production; the organic s

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0035] Example 1

[0036] 1000g marigold oleoresin (total lutein fatty acid ester content is 31.8%) is mixed with a mixed solvent consisting of 5000ml food grade absolute ethanol and 3000ml n-hexane, heated to 80°C under reflux and stirred until a homogeneous solution is formed. Keep the temperature at the temperature for 3 hours, and then slowly reduce the solution temperature to 25°C within 2.5 hours, keep the temperature for crystallization, and separate the obtained crystals with a centrifuge. The mass of the intermediate obtained is 401g. The crystalline solid contains lutein. The fatty acid ester content is 71% (weight percentage content). After saponification, the samples were tested by HPLC. Among them, all-trans lutein accounted for 92%, zeaxanthin accounted for 7%, and the rest were a small amount of cis-isomer and a small amount of other carotenoids.

[0037] Mix the obtained 400.0g intermediate crystals with 1000ml n-hexane, and after stirring, the temperature is raise...

Example Embodiment

[0040] Example 2

[0041] 1000g marigold oleoresin (the total lutein fatty acid ester content is 22.7%, of which the proportion of all-trans lutein is 72.7%) is mixed with a mixed solvent composed of 5600ml food grade absolute ethanol and 2000ml n-hexane, and the temperature is raised to 80 Reflux and stir at ℃ until a homogeneous solution is formed. Keep the temperature at this temperature for 5.0hr, then slowly reduce the solution temperature to 30℃ within 2.0 hours, keep the temperature for crystallization, and separate the crystals obtained by pressure filtration. The quality of the intermediate obtained is 392.5g, the content of lutein fatty acid ester in this crystalline solid is 68.5% (weight percentage content) after UV detection. The samples were saponified and tested by HPLC. Among them, all-trans lutein accounted for 90.5%, zeaxanthin accounted for 8.3%, and the rest were a small amount of cis-isomer and a small amount of other carotenoids.

[0042] The obtained 390.0g ...

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PUM

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Abstract

This invention discloses a method for separating and purifying high-content xanthophyl fatty acid ester from tagetes oleoresin. The content of xanthophyl fatty acid ester obtained is 70-80%, wherein the content of all-trans xanthophyl ester is 90-95%. The method has such advantages as low solvent residue, high yield and simple operation, and is suitable for mass production.

Description

Technical field: [0001] The invention belongs to the technical field of phytochemistry. Specifically, it relates to a method for separating and purifying high-content lutein fatty acid ester from marigold oleoresin. Background technique: [0002] Carotenoids are a class of yellow to red substances widely found in nature. In different fruits and vegetables, there are different kinds of carotenoids, such as beta-carotene in carrots, lutein in marigold flowers, zeaxanthin in strawberries, lycopene in tomatoes, capsicum plants The contents of capsanthin and capsanthin in turmeric are relatively high. However, there are more carotenoids in some dark fruits and vegetables, egg yolks, some fish shells, birds, seaweed and bacteria, and the content of lutein is also higher. [0003] Several animal and human trials in recent years have shown the beneficial effects of carotenoids. In general, carotenoids may be divided into two subcategories, namely, relatively strong polar xanthop...

Claims

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Application Information

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IPC IPC(8): C07C403/24C07C403/00A23L1/30
CPCC09B67/0096C09B61/00A23L1/2753C07C403/24A23L1/3002A23L5/44A23L33/105
Inventor 许新德陈伯秋周迪叶双明黄生聪邵斌
Owner 浙江可明生物医药有限公司
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