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Method for separating and purifying fatty acid ester of lutein in high content from resin of marigold oil

A fatty acid ester and lutein technology, applied in the field of phytochemistry, can solve the problems of unfavorable industrial production, residue, high solvent, etc., and achieve the effect of easy large-scale industrial production, suitable for large-scale industrial production, and high biological activity

Active Publication Date: 2006-12-06
浙江可明生物医药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] Thereby, there is following shortcoming in above-mentioned method, for example: the yield of product is not high, and purity is not enough; Large, unfavorable to industrial production; the organic solvent used in the method has a high boiling point, it is difficult to completely remove it in subsequent treatment, and there is a high solvent residue, which is difficult to apply to industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] 1000g marigold oleoresin (total lutein fatty acid ester content is 31.8%) is mixed with the mixed solvent that 5000ml edible grade dehydrated alcohol and 3000ml n-hexane are formed, is heated up to 80 ℃ and refluxes and stirs, until forming homogeneous solution, here Keep warm at high temperature for 3 hours, then slowly lower the temperature of the solution to 25°C within 2.5 hours, keep warm and crystallize, and separate the obtained crystals with a centrifuge to filter and separate the obtained crystals. The fatty acid ester content is 71% (weight percentage content). The sample was saponified and tested by HPLC, wherein all-trans lutein accounted for 92%, zeaxanthin accounted for 7%, and the rest were a small amount of cis-isomers and a small amount of other carotenoids.

[0037] Mix 400.0g of intermediate crystals obtained with 1000ml of n-hexane, heat up to 70°C and reflux for 2.0 hours under stirring, then slowly cool down to 10°C to crystallize, slowly add 80ml ...

Embodiment 2

[0041] 1000g marigold oleoresin (total lutein fatty acid ester content is 22.7%, wherein all-trans lutein ratio is 72.7%) mixes with the mixed solvent that 5600ml edible grade dehydrated alcohol and 2000ml normal hexane form, is warming up to 80 Reflux and stir at ℃ until a homogeneous solution is formed, keep at this temperature for 5.0hr, then slowly lower the solution temperature to 30℃ within 2.0 hours, keep warm and crystallize, and separate the obtained crystal by pressure filtration method, the obtained intermediate quality is 392.5g, detected by UV, the content of lutein fatty acid ester in this crystalline solid is 68.5% (weight percentage content). The sample was saponified and tested by HPLC, wherein all-trans lutein accounted for 90.5%, zeaxanthin accounted for 8.3%, and the rest were a small amount of cis-isomers and a small amount of other carotenoids.

[0042] Mix 390.0g of intermediate crystals obtained with 1500ml of n-hexane, and heat up to 70°C under stirrin...

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PUM

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Abstract

This invention discloses a method for separating and purifying high-content xanthophyl fatty acid ester from tagetes oleoresin. The content of xanthophyl fatty acid ester obtained is 70-80%, wherein the content of all-trans xanthophyl ester is 90-95%. The method has such advantages as low solvent residue, high yield and simple operation, and is suitable for mass production.

Description

Technical field: [0001] The invention belongs to the technical field of phytochemistry. Specifically, it relates to a method for separating and purifying high-content lutein fatty acid ester from marigold oleoresin. Background technique: [0002] Carotenoids are a class of yellow to red substances widely found in nature. In different fruits and vegetables, there are different kinds of carotenoids, such as beta-carotene in carrots, lutein in marigold flowers, zeaxanthin in strawberries, lycopene in tomatoes, capsicum plants The contents of capsanthin and capsanthin in turmeric are relatively high. However, there are more carotenoids in some dark fruits and vegetables, egg yolks, some fish shells, birds, seaweed and bacteria, and the content of lutein is also higher. [0003] Several animal and human trials in recent years have shown the beneficial effects of carotenoids. In general, carotenoids may be divided into two subcategories, namely, relatively strong polar xanthop...

Claims

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Application Information

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IPC IPC(8): C07C403/24C07C403/00A23L1/30
CPCC09B67/0096C09B61/00A23L1/2753C07C403/24A23L1/3002A23L5/44A23L33/105
Inventor 许新德陈伯秋周迪叶双明黄生聪邵斌
Owner 浙江可明生物医药有限公司
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