Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Ferment method for producing 2.3-butanediol

A butanediol, fermentation and cultivation technology, applied in the 2 field, can solve the problems of low conversion rate, long fermentation cycle, low yield of 2,3-butanediol, etc.

Inactive Publication Date: 2006-12-27
EAST CHINA UNIV OF SCI & TECH
View PDF0 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The technical problem to be solved in the present invention is to disclose a method for producing 2,3-butanediol by fermentation, so as to overcome the problems of long fermentation period, low yield of 2,3-butanediol and low conversion rate in the prior art. defect

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Ferment method for producing 2.3-butanediol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] The following are percentages by weight.

[0051] Yeast powder 0.5%, peptone 2%, citric acid 1%, MnSO 4 0.007%, KH 2 PO 4 0.05%, MgSO 4 0.05% add 800ml water to dissolve;

[0052] Weigh FeSO 4 ·7H 2 O 0.002%, add 20ml of water to dissolve, add 3ml of hydrochloric acid with a concentration of 1 / 4 (v / v) to help dissolve, make the solution clearer, and filter with a sterile filter membrane;

[0053] Mix the above medium, adjust to 7.20 with 5mol / l NaOH solution, add water to 1L, and sterilize;

[0054] The sterilized sucrose is mixed with the above-mentioned cooled medium to obtain 9% sucrose, namely to obtain the fermentation medium.

Embodiment 2

[0056] (1) Seed culture of Serratia marcescens:

[0057] The culture time is 12 hours, and the culture temperature is 28 DEG C. The components and weight content of the culture medium are: glucose 1%; yeast powder 0.1%; peptone 0.2%; (NH 4 ) 2 SO 4 0.6%;K 2 HPO 4 1%; NaCl 0.05%; MgSO 4 0.05%; adjust pH 7.2 with NaOH.

[0058] (2) Fermentation culture:

[0059] The volume of the initial fermentation medium is 1.8L, and the composition of the initial medium used is as follows

[0060] Sucrose 9%

[0061] Peptone 2%

[0062] Yeast powder 0.5%

[0063] Citric acid 1%

[0064] MnSO 4 0.007%

[0065] NaH 2 PO 4 0.05%

[0066] FeSO 4 0.002%

[0067] MgSO 4 0.05%

[0068] The concentration of each component of the feeding solution is:

[0069] Feed 1 sucrose aqueous solution: 500g / 600ml;

[0070] Feed 2 Industrial amino acid powder aqueous solution: 40g / 200ml; (added in the second stage)

[0071] Inoculate the Serrat...

Embodiment 3

[0077] Production of 2,3-butanediol by Serratia marcescens using the original medium.

[0078] Medium composition:

[0079] Glucose 5%, maltose extract 0.3%, yeast powder 0.3%, peptone 0.5%, and the pH was adjusted to 7.2 before sterilization.

[0080] Insert the Serratia marcescens seed liquid with the inoculum size of 5% (volume ratio), 30 ℃ shaker, 200rpm cultivate, with NaOH and H 2 SO 4 The pH of the cultivation process was controlled to be 6.0. After 42 hours, the sugar was consumed, and the 2,3-butanediol production was measured to be 15 g / L.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for fermenting and producing 2,3-butanediol. Inoculate the Serratia marcescens seed liquid into the culture medium, introduce air, stir, and control the RQ between 0.9 and 1.2; then add the product promotion factor, add sucrose aqueous solution and amino acid aqueous solution, and control the RQ between 1.5 and 1.8 Between; finally added sucrose aqueous solution to control the RQ between 1.9 and 2.2, and fermented and cultured to obtain the fermentation culture liquid containing 2,3-butanediol. The method of the present invention adopts the static optimization method combining single factor experiment, orthogonal experiment and response surface experiment, optimizes the culture medium, and uses RQ to regulate and produce the production of 2,3-butanediol, and through stage-by-stage regulation, makes The output of 2,3-butanediol has been greatly increased, the output of 2,3-butanediol has reached more than 107g / L, the conversion rate has reached more than 0.45g / g suc, and the production capacity has reached more than 2.91g / L·h, which has industrialization promotion Applied prospects.

Description

technical field [0001] The invention relates to a production method of 2,3-butanediol, in particular to a method for fermenting and producing 2,3-butanediol by Serratia marcescens. technical background [0002] 2,3-butanediol (2,3-butanediol, referred to as BD, the same below) is widely used in various fields such as chemical industry, food and aerospace. Its calorific value is 27200kJ / kg, which is close to that of ethanol (29100kJ / kg). It can be used as a fuel, and can also be used to prepare polymers, inks, perfumes, antifreeze agents, aromatherapy agents, humidifiers, explosives and chiral carriers for medicines. Wait. [0003] It is dehydrated into methyl ethyl ketone, which can be used as a solvent for resins and paints; dehydrated into butadiene, which can be used for synthetic rubber; it can also replace 1,4-butanediol for the synthesis of polyester and polyurethane; Methyl ethyl ketone is condensed and hydrogenated to form octane, which can be used to produce high-...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/18C12R1/425
Inventor 沈亚领魏东芝曹学李元芳朱虎王学东
Owner EAST CHINA UNIV OF SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com