Preparation of cotton-seed polypeptide with anti-oxidant function

A technology of anti-oxidation and production method, which is applied in the field of nutritious food and functional, can solve the problems of loss of activity of hydrolyzate, different characteristics of hydrolyzate, time consumption of energy and so on, so as to shorten the time of hydrolysis and improve the utilization rate of enzyme , The effect of low production cost

Inactive Publication Date: 2007-01-17
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many techniques for enzymatically hydrolyzing protein. Some use a single type of enzyme to hydrolyze, which takes a long time and consumes energy. As the hydrolysis time prolongs, the activity of the hydrolyzate gradually loses; some use multiple types of Enzymes are hydrolyzed at the same time, but by choosing different types of enzymes and their combinations, and using different hydrolysis conditions, the hydrolysates obtained are different, and the properties of the hydrolysates are also different;

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1, the production of the dry powder of cottonseed polypeptide with antioxidant effect

[0017] Take 20 kg of cottonseed protein isolate with a protein content of 60%, add 60 kg of distilled water, grind it into a fine and uniform cottonseed protein isolate slurry with a colloid mill, add 320 kg of distilled water, put it into the reaction tank, and use 1mol / L Sodium hydroxide solution to adjust its pH value to 7.9, add 1 kg of alkaline protease labeled Alcalase (in the state of continuous stirring, and heat to 54 ° C for hydrolysis, constantly use 1mol / L sodium hydroxide solution in the hydrolysis process Adjust and maintain the pH value of 8.0, keep the temperature at 54°C, and continue the hydrolysis for 1 hour. After the first hydrolysis, adjust its pH value to 7.0 with a molar concentration of 1mol / L hydrochloric acid solution, and adjust the temperature to 50°C. In the state of constant stirring, add the label Flavourzyme TM 1.2kg of flavor protease, in...

Embodiment 2

[0018] Embodiment 2, the production of the dry powder of cottonseed polypeptide with antioxidant effect

[0019] Take 18 kg of cottonseed protein isolate with a protein content of 70%, add 72 kg of distilled water, grind it into a fine and uniform cottonseed protein isolate slurry with a colloid mill, then add 280 kg of distilled water, put it into the reaction tank, and use 1mol / L Sodium hydroxide solution to adjust its pH value to 8.0, add 0.8 kg of alkaline protease labeled Alcalase under continuous stirring, and heat to 55°C for hydrolysis, and continuously use 1mol / L sodium hydroxide solution during the hydrolysis process Adjust and maintain the pH value at 7.9, maintain the temperature at 55° C., and continue the hydrolysis for 1.5 hours. After the first hydrolysis, adjust the pH value to 7.1 with a hydrochloric acid solution with a molar concentration of 1 mol / L, adjust the temperature to 49°C, and add Flavourzyme under constant stirring. TM 0.8kg of flavor protease, d...

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PUM

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Abstract

A process for preparing the antioxidizing cottonseed polypeptide powder used as the nutrient intensifier for improving immunity includes such steps as mixing cottonseed protein with distilled water, grinding, regulating pH value and temp, adding alkaline proteinase, enzymolyzing, adding flavouring proteinase, regulating pH value and temp, enzymolyzing, boiling for deactivating enzyme, cooling, filtering, vacuum concentrating, and spray drying.

Description

technical field [0001] The invention relates to a treatment method of cottonseed protein, in particular to a method for preparing a dry polypeptide powder with anti-oxidation function by using the cottonseed protein. The dry polypeptide powder belongs to functional and nutritious food. Background technique [0002] Cotton is one of the important crops in the world. Cottonseed is rich in more than 30% protein. Its amino acid composition is complete, the ratio is reasonable, and its nutritional value is close to that of bean protein. It is a very important plant protein resource. Biologically active peptides are polypeptides with amino acid sequences, which have special physiological activities on the life activities of biological organisms. Certain low-peptides can not only provide the nutrients needed for human growth and development, but also have the functions of preventing and treating diseases and regulating human physiological functions, such as lowering blood pressure,...

Claims

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Application Information

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IPC IPC(8): A61K38/16A61K38/48A61P39/06A23L1/305A23L33/18
Inventor 高海燕倪晋仁贾征左航
Owner PEKING UNIV
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