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Peptide for promoting insulin release of frog and its use in production of drugs

An insulin and stinky frog technology, applied in the field of biomedicine, can solve the problems of islet β-cell failure, large side effects, and cannot cover metabolic defects, etc., and achieves the effect of no plasma coagulation activity and promoting insulin release.

Inactive Publication Date: 2007-01-17
KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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Problems solved by technology

However, due to the large side effects of sulfonylurea drugs, long-term use will lead to the failure of pancreatic β cells due to excessive secretion of insulin. Among diabetics taking such drugs, 10% of patients will switch to insulin every year due to treatment failure. other drugs
Moreover, current pharmacological research and treatment methods do not yet cover all the metabolic defects involved in the disease

Method used

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  • Peptide for promoting insulin release of frog and its use in production of drugs

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Embodiment Construction

[0017] The substantive content of the present invention is further described below with embodiment, but content of the present invention is not limited thereto.

[0018] Preparation of insulin release-promoting peptides from fingerless frogs:

[0019] Clean the living fingerless stink frog with water, put it in a glass container with a cover, add anhydrous ether (1 liter of volume plus 1 ml of anhydrous ether) dropwise, and seal the container for 3-5 minutes, and a large amount of foam can be seen. The substance is secreted from the skin of the brown-spotted frog, and the secretion is collected, centrifuged (10000rpm, 20 minutes), freeze-dried and stored.

[0020] In the first step, Sephadex G-50 gel filtration column chromatography, 300 mg of the lyophilized powder of the skin secretion of the stink frog skin secretion obtained by the above method was dissolved in 5 ml of purified water, and centrifuged at 10,000 rpm at 4°C for 10 min to remove the precipitate. SephadexG-50 ...

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Abstract

A non-finger scent frog insuline releasing stimulating peptide and use in pharmacy are disclosed. The stimulating peptide is prepared by separating from non-finger scent frog skin secretory liquid, purifying, determining its sequence and synthesizing polypeptide. It belongs to a single-chain polypeptide with molecular weight 2632.3 and isoelectric point 10.32. Polypeptide total sequence is phenylalanine-leucine-proline-leucine-lecucine-lactamic acid-glycine-leucine-lactamic acid-lactamic acid-agedoite-phenylalanine-leucine-proline-lysine-leucine-phenylalanine-cystecoside acid-lysine-isoleucine-threonine-arginine-lysine-glycine. It can improve insuline release, has excellent hemolytic and plasma clotting activities. It can be used to treat diabetes.

Description

Technical field: [0001] The invention relates to an insulin-releasing-promoting peptide of the fingerless frog and its application in pharmacy, belonging to the technical field of biomedicine. Background technique: [0002] Diabetes mellitus is a multiple endocrine and metabolic disease caused by multiple genetic and environmental factors with sugar, fat, and protein metabolism disorders, resulting in multiple system and multiple organ dysfunction. Statistics show that in my country in the 1980s, it was <1%, and it has risen to 3.6-5% at present, of which more than 90% (about 38 million people) belong to type II diabetes. As of 2000, there were 135 million people with diabetes in the world, and it is expected to increase to 299 million in 2025. Diabetes itself is not terrible, what is terrible is the complications, which include cancer, sleep apnea, depression, myocardial infarction, peripheral vascular disease, hypertension and the most common type II diabetes. Type II...

Claims

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Application Information

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IPC IPC(8): C07K14/435A61K38/17A61P3/10
Inventor 赖仞李建许梁建国徐学清李东升李宗杰
Owner KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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