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Method for preparing paris polyphylla total saponin and its use

A technology of total saponins and glutathione is applied in the field of preparation of effective parts of erythromycin-total saponins, can solve the problems of excessive ethanol consumption, large ethanol consumption, complicated process steps, etc., and achieves reduction of ethanol consumption, production cost, and preparation The effect of process simplification

Inactive Publication Date: 2007-01-24
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The simple resin method is to concentrate and centrifuge the extract of the plant, and the obtained supernatant is passed through a macroporous resin column to prepare the total saponins of the plant. The defect of this method is that: due to the poor water solubility of the total saponins of the plant, a large amount of The total saponins are precipitated when the extract is concentrated, resulting in only a part of the total saponins remaining in the supernatant after the centrifugation of the concentrated solution, so that the total saponins of Parisia saponins are prepared by this method, and the extraction recovery rate of the active ingredients is only less than 50%.
Membrane filtration combined with resin adsorption method improves the low recovery rate of active ingredients in simple resin method, but the process steps are cumbersome, and the amount of ethanol used in the production process is large. With the soaring energy prices, the complicated process and excessive ethanol consumption make large industries Significantly higher cost of production
In addition, the use of organic solvents such as acetone during decolorization in this process also increases the cost and danger of production to a certain extent

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] It is 1 kg of Chonglou decoction pieces, add 6 times the amount of 60% ethanol, reflux extraction 2 times, each extraction time is 1 hour, combine the ethanol extract, and centrifuge the combined ethanol extract (rotating speed 4000 rpm, time 30 minutes ), separate the precipitate and supernatant, concentrate the supernatant to 0.25g crude drug / ml, let stand overnight and then centrifuge to obtain supernatant A and precipitate A'. Add deionized water equivalent to 1 times (V / W) of the crude drug used in the extraction to the precipitate A', stir and wash, then centrifuge again (rotating speed 4000 rpm, time 30 minutes), pour out the supernatant to obtain Supernatant B and precipitation B'; combined supernatant A and B, the supernatant after combining A and B was passed through a chromatographic column equipped with D101 type macroporous adsorption resin (non-polar), and the supernatant was pressed The ratio of the total saponins of Pachyphylla saponins to the dry resin ...

Embodiment 2

[0045] Get Chonglouyin coarse powder 1kg, add 12 times the amount of 95% ethanol, reflux extraction 3 times, each extraction time is 3 hours, merge the ethanol extract, and the ethanol extract after merging is centrifuged (rotating speed 10000 rpm, time 90 minutes); separate the precipitate and supernatant, concentrate the supernatant to 1.0g crude drug / ml, let stand overnight and then centrifuge to obtain supernatant A and precipitate A', add the equivalent of The deionized water with 5 times (V / W) amount of crude drug was stirred, washed, and then centrifuged again (rotating speed 10000 rpm, time 90 minutes), and the supernatant was poured out. Obtain supernatant B and precipitate B'; merge supernatant A and B, the supernatant after combining A and B is passed through the chromatographic column equipped with AB-8 type macroporous adsorption resin (weak polarity), and put on The ratio of supernatant liquid to dry resin is 1:10 according to the amount of total saponins of Pach...

Embodiment 3

[0047] Take 1 kg of Chonglou decoction pieces, add 8 times the amount of 70% aqueous alcohol, reflux extraction twice, each extraction time is 2 hours, combine the ethanol extract, filter; concentrate the filtered ethanol extract to 0.5g crude drug / ml , stand overnight, filter to obtain supernatant A and precipitate A', add deionized water equivalent to 2 times the amount of crude drug used for extraction (V / W) to precipitate A', stir, wash, centrifuge, and pour out supernatant, supernatant B and precipitation B' are obtained; supernatant A and B are combined, and the supernatant after merging A and B is passed through a D101 type macroporous adsorption resin (non-polar) chromatographic column, The ratio of the supernatant to the dry resin is 1:7 according to the total saponins of Pachyphylla saponins, the upper column flow rate is 2BV / h, and the diameter-to-height ratio of the resin is 1:5; the resin is washed with water for 5BV, and the elution flow rate is 3BV / h. Discard th...

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PUM

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Abstract

The present invention discloses preparation process and use of Chinese paris rhizome total saponin as the effective component. The preparation process of the present invention is one simplified one comprising alcohol extraction, water precipitation, and deionized water washing and depositing. The preparation process has lowered alcohol consumption, obviously reduced production cost, no use of acetone, high effective component yield, high product purity and stable quality. The present invention also relates to the application of Chinese paris rhizome total saponin in preparing externally applied medicine for diminishing inflammation, eliminating swelling and relieving pain, antiviral medicine, and anti-tumor medicine.

Description

technical field [0001] The invention belongs to the field of pharmacy, and in particular discloses a preparation method and application of total saponins, the effective part of the plant. Background technique [0002] Chonglou is the dry rhizome of Paris polyphylla Smith var.yunnanensis (Franch.) Hand.Mazz. and P. polyphylla Smith var.chinensis (Franch.) Hara of Liliaceae plants. In my country, Chonglou is the main component of many well-known Chinese patent medicines, such as Yunnan Baiyao, Jidesheng Sheyao Tablets, and Gongxuening Capsules. However, these medicines are basically prepared by combining Chonglou with other medicines and then directly preparing them with water or ethanol extracts, retaining the traditional Chinese medicine preparation method. The total saponins of Chonglou are the main active ingredients of the multiple pharmacological effects of Chonglou. The effective part of Chonglou is to enrich the total saponins to more than 50% by applying modern scien...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/896A61P29/00A61P31/12A61P35/00C07H1/08
Inventor 张琳孙治国田景奎梁波李凌军
Owner ZHEJIANG UNIV
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