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Method of extracting and purifying sphenomeris chinensis orientin and vitexin

A technology of orientin and purification method, which is applied in chemical instruments and methods, organic chemistry, medical raw materials derived from ferns/filamentous plants, etc., and can solve the problems of numerous operation steps, high toxicity, and high price

Inactive Publication Date: 2007-02-07
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing separation schemes for orientin or vitexin all adopt multiple column chromatography on silica gel, polyamide, macroporous resin, etc., and the operation steps are numerous, and the eluent contains chloroform, benzene, acetone, ethyl acetate, etc. Chloroform and benzene are highly toxic, and the prices of acetone and ethyl acetate are relatively high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0023] (1) Soak the aerial part of S. chinensis with 70% ethanol for 2 times, and the volume of ethanol added is 15 times of the weight of the medicinal material, and each time of leaching for 7 days. The extract was filtered and concentrated, and the ethanol was evaporated to obtain a crude extract.

[0024] (2) The crude extract was sequentially extracted 4 times with petroleum ether, 3 times with ethyl acetate, and 5 times with n-butanol, and the n-butanol extract was concentrated under reduced pressure at 60°C to obtain the n-butanol fraction.

[0025] (3) The concentrated n-butanol part is placed in a refrigerator at 4°C for 10 days, and yellow granular precipitates precipitate out. The precipitates are slightly soluble in solvents such as water, ethanol, ethyl acetate, and chloroform, and are difficult to separate and purify by recrystallization. .

[0026] (4) Prepare the precipitate into an aqueous suspension, add 1% NaOH dropwise until the solution is clear, add 50 t...

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PUM

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Abstract

The invention discloses an extracting and purifying method of fern pteroside and vitexin, which is characterized by the following: obtaining rough flavone educt from fern; using buck to dissolve; neutralizing through acid; obtaining the object product by over 98% purity through inverse-phased silica gel column chromatography.

Description

technical field [0001] The invention relates to a method for separating and purifying active ingredients from plants, in particular to extracting and purifying flavonoid compounds orientin and vitexin from the aerial part of S. Background technique [0002] Stenoloma chusanum (Linn.) Ching is a plant of the genus Stenoloma chusanum (Lindsaeaceae) in the family Lindsaeaceae. Black fern has a detoxification effect on arsenic poisoning and dimethoate poisoning in mice, prevents and treats CCl4 liver damage in mice, and can treat livestock hepatitis. It is also used clinically to treat diarrhea, intractable ulcers of lower limbs, chronic renal failure and other diseases. Recently, the author has isolated flavonoids such as Orientin and Vitexin from the aerial part of C. [0003] Orientin in plants is usually extracted with ethanol, and then extracted with n-butanol. The n-butanol extract is obtained by silica gel column chromatography and eluted with chloroform-methanol solvent...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H1/08A61K36/11A61K127/00
Inventor 任冰如夏冰李维林吴菊兰张涵庆
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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