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Methods for reducing the effects of stress on skin condition

A technology for skin inflammation and chronic stress, applied to skin diseases, medical preparations containing active ingredients, pharmaceutical formulas, etc., can solve problems such as poor knowledge of skin appearance

Inactive Publication Date: 2007-02-14
UNILEVER NV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But little is known about the effects of this stress on skin appearance and the underlying biological mechanisms

Method used

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  • Methods for reducing the effects of stress on skin condition
  • Methods for reducing the effects of stress on skin condition
  • Methods for reducing the effects of stress on skin condition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0115] Example 1: Effects of Chronic Stress on the Inflammatory State of Endothelial Cells

[0116] Summary of Experimental Methods

[0117] An in vitro model has been developed to study the effect of chronic stress on the inflammatory state of endothelial cells.

[0118] a. Let the cells in 6 wells (9.5cm 2 ) plate growth.

[0119] b. Pretreatment with synthetic glucocorticoid (dexamethasone) was added every day for 5 days, so that the cells were subjected to "chronic stress".

[0120] c. At time point TO, harvest tissue culture supernatant and cell pellet.

[0121] d. Cells were subjected to oxidative stress with 1 [mu]M phorbol myristate acetate (PMA).

[0122] e. Tissue culture supernatants and cell pellets were harvested 6 hours, 24 hours and 48 hours after PMA treatment (T6, T24 and T48, respectively).

[0123] f. All tissue culture supernatants were assayed for lactate dehydrogenase (LDH) to measure cytotoxicity and interleukin-6 synthesis.

[0124] g. All the cel...

Embodiment 2

[0175] Example 2: Effects of chronic stress on dermal matrix remodeling

[0176] The dermal matrix component of the skin is mainly composed of elastic fibers, collagen fibers, proteoglycans and other glycoproteins. This connective tissue complex acts as a scaffolding for the dermis to which cells, such as fibroblasts, can attach. The major protein component of such tissues is collagen, a fibrous protein of heterogeneous nature. Type I collagen is the predominant structural protein in the skin, and the appearance of aging skin is largely attributable to the breakdown of type I collagen. Normal fibril formation requires multiple post-translational modifications, including the proteolytic conversion of precursor procollagen to collagen. Therefore, the estimated amount of procollagen I can reflect the amount of collagen I molecules to be synthesized. Indeed, the histological features of damaged aged skin typically include flattened, hardened, disorganized bundles of collagen fi...

Embodiment 3

[0213] Example 3: Identification of drugs that inhibit glucocorticoid-mediated effects on skin cells-inflammation

[0214] Materials and Methods

[0215] The same method as described in Example 1 was used, except that various test drugs were added to the cells during pretreatment (see Table 1).

[0216] drug

source

22-OH-cholesterol

Alliin

caffeic acid

Ciglitazone

bisabolic acid

curcumin

Geldanamycin

Ginsenoside Rb1

Ginsenoside Rc

Kyolic brand garlic

lovastatin

Okadaic acid

Pycnogenol (pycnogenol)

resveratrol

rosemary extract

Theanine

WY14643

Sigma H9384

Aldrich 74264

Sigma C0625

Calbiochem 230950

C. Rawlins, Unilever Research

Colworth (method below)

Sigma C7727

Sigma G3381

Sigma G0777

Sigma G0902

Quest Vitamins Ltd, Birmingham, UK

Sigma M2147

Sigma O9381

Nature's Aid, Preston, UK

Sigma R5010

A&E Co...

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PUM

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Abstract

A method is provided for reducing the effects of psychologically-mediated stress on the skin of a human or animal which method comprises administering to the individual a composition capable of inhibiting glucocorticoid-induced chronic stress in a dermal cell or a cell involved in skin inflammatory responses. Also provided are compositions for use in such methods and assay methods for identifying suitable compositions.

Description

field of invention [0001] The present invention relates to methods of reducing the effects of chronic psychological stress on skin conditioning. The invention also relates to compounds useful in this method and assay methods for identifying suitable compounds. Background of the invention [0002] Longitudinal studies have shown that neuroendocrine stress due to everyday life is a major driver of age-related disorders. Plus, multiple consumer studies have shown that psychological stress is critical to an individual's well-being. But little is known about the effects of this stress on skin appearance and the underlying biological mechanisms. Summary of the invention [0003] We developed a model system that mimics the effects of chronic stress and used this system to study the effects of chronic stress on the skin. Using this model, we show that chronic stress, represented by chronic treatment of skin cells with glucocorticoids, results in a reduced ability of the skin to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/425A61K31/366A61K31/35A61K31/38A61K38/22A61K36/258A61K36/815A61K31/704A61K31/12A61K31/565A61P17/00
CPCA61K31/202A61K36/258A61P17/00A61P17/02A61P17/04A61P17/06A61K2300/00
Inventor K·E·巴雷特S·P·格兰杰G·詹金斯L·J·温赖特
Owner UNILEVER NV
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