Chemical luminescent analysis reagent kid for quantitatively detecting trilute
A technology for orthocyanidin chemistry and triiodothyroidism, which is applied in the field of clinical blood immune detection, can solve the problems of radioactive contamination of operators, complex detection equipment, and short storage period, etc., to achieve enhanced detection signals, increased uniformity, and improved results. The effect of improved reproducibility
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[0037]Chemiluminescence assay kit for quantitative detection of triiodothyronine, mainly composed of (1) opaque polystyrene plate coated with rabbit anti-triiodothyronine antibody, (2) hormone-free serum or analysis buffer Triiodothyronine series standard prepared by adding pure triiodothyronine as matrix, (3) triiodothyronine labeled with horseradish peroxidase, (4) luminescent substrate A solution and (5) luminescent substrate B solution are composed. , The luminescent substrate A solution is made of high-efficiency luminescent agent, compound enhancer and amino acid, and the luminescent substrate B solution is made of amino acid oxidase and stabilizer.
[0038] The opaque polystyrene plate coated with rabbit anti-triiodothyronine antibody was coated by the bridge-antibody method, first excessively coated with goat anti-mouse IgG, and then indirectly coated with anti-triiodothyronine monoclonal antibody; Root peroxidase-labeled triiodothyronine can be diluted with triiodoth...
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