Chemical luminescent analysis reagent kid for quantitatively detecting trilute

A technology for orthocyanidin chemistry and triiodothyroidism, which is applied in the field of clinical blood immune detection, can solve the problems of radioactive contamination of operators, complex detection equipment, and short storage period, etc., to achieve enhanced detection signals, increased uniformity, and improved results. The effect of improved reproducibility

Active Publication Date: 2007-03-07
AUTOBIO DIAGNOSTICS CO LTD
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Problems solved by technology

Because it must use radioactive markers, the detection equipment is complex, and special radioactive detectors must be used to determine the detection results
At the same time, there are also radioactive pollution and injuries to operat

Method used

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Embodiment Construction

[0037]Chemiluminescence assay kit for quantitative detection of triiodothyronine, mainly composed of (1) opaque polystyrene plate coated with rabbit anti-triiodothyronine antibody, (2) hormone-free serum or analysis buffer Triiodothyronine series standard prepared by adding pure triiodothyronine as matrix, (3) triiodothyronine labeled with horseradish peroxidase, (4) luminescent substrate A solution and (5) luminescent substrate B solution are composed. , The luminescent substrate A solution is made of high-efficiency luminescent agent, compound enhancer and amino acid, and the luminescent substrate B solution is made of amino acid oxidase and stabilizer.

[0038] The opaque polystyrene plate coated with rabbit anti-triiodothyronine antibody was coated by the bridge-antibody method, first excessively coated with goat anti-mouse IgG, and then indirectly coated with anti-triiodothyronine monoclonal antibody; Root peroxidase-labeled triiodothyronine can be diluted with triiodoth...

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Abstract

This invention relates to quantitatively triiodothyronine chemical light analysis agent box, which belongs to clinical blood immune testing technique field and comprises the following parts: non-transparent polyphony lacetylene board with the gland antigen, prostate antigen series standard product, enzyme mark gland specific antigen, lighting bottom object A liquid composed of effective light agent, compound strengthening agent and amino acid and B liquid composed of amino acid oxidase and stabilizer.

Description

technical field [0001] The invention belongs to the technical field of clinical blood immune detection, in particular to a kit for quantitatively detecting the content of triiodothyronine (T3) by an enzymatic chemiluminescence method. Background technique [0002] The human thyroid is an important part of the endocrine system, and thyroid hormones participate in many important physiological reactions. They play an important role in regulating human growth and development, cell metabolism and hormone balance in the body, maintaining metabolism, skeletal muscle and the development of various organ systems. Most of the thyroxine (T4) and 3,5,3'-thyronine (T3) in the blood circulation are combined with the plasma protein - thyroid-binding globulin (TBG). The concentration of T3 is much lower than that of T4, but it has stronger physiological activity, so the concentration of triiodothyronine (T3) in serum is an important parameter for evaluating thyroid function. T3 detection ...

Claims

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Application Information

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IPC IPC(8): G01N33/547G01N33/52G01N21/64G01N33/531
Inventor 付光宇陈晓玲张莹于鹏举项立红李晓霞
Owner AUTOBIO DIAGNOSTICS CO LTD
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